PURIFICATION OF GLUCOSE AND GALACTOSE SPECIFIC LECTINS FROM NIGELA SATIVA SEED

Abstract

ABSTRACTGlucose specific lectin (GL) and galactose specific lectin (GaL) werepurified from Nigela sativa at the first time in country. Purification stepsincluded fractionation with ethanol (50-75%), Dialysis and affinitychromatography in sephadex (G-50) column for (GL) specific lectin andsepharose (4B) Column for (GaL) specific lectin. Lectin were purified tohomogeneity as indicated by the presence of one band in polyacrylamid gelelectrophoresis in the absence of denaturing conditions. The molecular weight ofpurified lectins measurements by gel filtration suggest values of 25000 and50000 dalton for (GL) and (GaL) specific lectins respectively, and 25.000,50.000 dalton by Polyacrylamide gel (SDS) electrophoresis.Keywords: Glucose specific lectin, galactose specific lectin affinity chromatography,polyacrylamid gel.PDF created