INHIBITORY ACTION OF CINNAMON CLOVETREE AND PROPOLIS EXTRACTS AGAINST Melisscoccus Pluton THAT CAUSE EFB IN HONEYBEE

Abstract

Experimental isolation and purification of the bacteria Melissococcus pluton that cause European foulbrood disease (EFB), and antimicrobial activity of plant extracts were carried out in the laboratories of Biotechnology Center of Al-Nahrine University. Morphological, physiological and biochemical tests showed that the bacterium M. pluton was found in one sample of sum of 12 samples that were tested, with an infection incidence 8.33%. Hexan extracts of the Cinnamon (Cinnamomum zeylanicum), Clovetree Eugenia cavyophylata and alcohol extract of propolis were examined using the qualitative disc diffusion assay. Results were showed that the clovetree extract was more effective at concentrate 2500mg/disc on bacterial growth that caused zone of growth inhibition(19.75mm in diameter) compared with control treatment(6mm). Propolis and clovetree extracts (2500mg/disc) were much effective on growth of M.pluton compared with oxytetracycline, as it is considered the drug of choice for control EFB infection all over the world.