THE PHYSIOLOGICAL EFFECT OF GONADOTROPINS FOR INDUCTION OF SUPEROVULATION AND IN VITRO OOCYTE MATURATION AND FERTILIZATION IN MICE

Abstract

ABSTRACTIt was considered that the selection of appropriate superovulationprogram and in vitro maturation (IVM) by using culture media hasimportant steps for successful of assisted reproductive techniques for medicalor economical or research purposes. Therefore, this study was designed to: 1-Determination the best superovulation program in mice, and 2-Determination the effect of using of culture media on IVM and in vitrofertilization (IVF) and embryonic development later in mice.Four hundred and twenty females were divided into seven groups andinjected with (10 or 15 or 30) IU dose of either pregnant mare serumgonadotropin (PMSG) or human menopausal gonadotropin (hMG), inaddition to control group was injected with 0.2 ml of normal saline. Also,natural mating was achieved for fifty superovulated and fifty nonsuperovulated females. Furthermore, nine hundred and ninety five oocyteswere incubated within TCM-199 and RPMI-1640 for IVM. Then, maturedoocytes and active spermatozoa were incubated withinMEM for IVF. Resultsof superovulation programs, number of births, percentages of IVM and IVFwere registered.Results of the present study appeared that the injection of PMSG hasresults better than the hMG. Also, injection of dose 10 IU has results betterthan the doses 15 IU and 30 IU. Non significant differences in the number ofbirths between superovulated and non superovulated females were reported.Also, insignificant differences in IVM between TCM-199 and RPMI-1640were noticed. It was observed that the MEM has good ability for IVF. Fromthe results of this study, it was concluded that the injectionof 10 IU of PMSGfor female mouse was effective to induce superovulaton. Also, the importanceto use culture medium MEM for IVF of oocytes matured in vitro usingculture media TCM-199 and RPMI-1640.