Study the effect of some factors in the induction of callus from the plant beans

Abstract

The best microspores developmental stage was determined by adopting a new rapidtaining method using orcein- HCl stain. It was found that the third stage was better the stagefor callus induction. For anther culture conditions the pretreatment temperature (3Cْ) wasapplied for 1-13days,basal MS media were used as liquid ( with or without ficoll ) and solid(with 0.8% agar or 0.5% agarose ), hormones with BA: 0.5 and 1mg/l and IAA: 0.1,0.5and1mg/l) or without hormones, activated charcoal (0.1,0.3 and 0.5%), colchicines(25mg/l),too different concentration from sucrose [3,4,5,6%].Casein hydrolysate , amino acidsand lighting conditions full darkness and light (white and red).The result showed that the pretreatment temperature gave less callus induction than thecontrol treatment. The solid media gave better callus induction than liquid media especiallywhen agarose was used. Whereas activated charcoal treatment and colchicine (added to themedia) did not induce callus formation and the sucrose (4%) gave better callus induction(33.3%). The results also revealed that the red light gave callus induction (5%) better than thewhite light and darkness.