Effect of collection techniques on recovery, and in vitro maturation of Black Iraqi Goats oocytes
Abstract
The study was conducted to know the effect of collection techniques on recovery and on in vitro maturation of goat oocytes. Oocytes were collected by two techniques aspiration and slicing of goat ovaries obtained from slaughter house. It was observed that aspiration showed a recovery rate of 76.94% (1401/1821) while slicing method showed a recovery rate of 69.93% (884/1264). There was a significant difference (P<0.05) between the two methods. Aspiration method showed a maturation rate of 40.68% of the recovered oocytes of good quality (1297/1401), While the slicing method showed a maturation rate of 30.84% of the recovered oocytes (198/642). There was a significant difference (P<0.05) in maturation rate between the aspiration and slicing methods.It was concluded from this study that the method of collection oocytes from ovaries in slaughter house effect on recovery and the maturation of oocyte in Black Iraqi Goat.
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