THE OPTIMUM CONDITIONS FOR ENDOGLUCANASE PRODUCTION FROM ASPERGILLUS FLAVUA

Abstract

ABSTRACTFifteen local fungal isolates produced endoglucanase (EC. 3.2.1.4) from differentareas belonged to five genera namely Eggplant, corn, millet, tomato, Wheat and soilswere subjected to endoglucanase production test, the isolate Aspergillus flavua wasselected based on its high production endoglucanase was reach 1 unit/ml. The optimalcondition for production endoglucanase using modify czapekdox contained 2%carboxyl methylcellulase, 0.2% ( NH4)2 HPO4, 0.5% MgSO4 2H2O, 1% K2HPO4, 0.5%KCL, 0.01%FeSO4 2H2O and optimum inoculia level was 106 spore /ml and the initialPH was 5 after 8 day incubation period was at 30°C and the production was reach4.95 unit/ml.