THE ANTIBACTERIAL ACTIVITY OF COLD AQUEOUS AND PIGMENT OF HIBISCUS ROSA SIENSIS EXTRACTS AGAINST GRAM POSITIVE AND NEGATIVE BACTERIA

Abstract

The antibacterial activity of aquaeous and purified pigment extracts of Hibiscus rosa siensis in concentration (20,50,100,200,250 mg) were tested against reference strains of Staphylococcus aureus and Esherichia coli . The preliminary qualitative tests showed that the two extracts have flavonoids, carbohydrates and glycosides ,while alkaloids found only in the aquaeous extract. Thin Layer Chromatography (TLC) showed the presence of anthocyanin pigment . Both extracts gave a clear activity against the tested strains with a minimal Inhibitory concentration reached to 20 mg/ml.