The Susceptibilities of Gardnerella vaginalis Isolates from Iraqi Hospitals Towards Various Antibiotics and New Mixed Ligand Complexes of 5,5-diphenyl-imidazolidine-2,4-dione with transition metals(II)
Abstract
Thirteen isolates of Gardnerella vaginalis isolates distinguished out of 250 of high vaginal swabs from female patients collected from Baghdad area hospitals, with an incidence of presence 5.2 %. These specimens belong to patients aged between 14 - 48, most of them where non-pregnant suffering from itching and vaginal discharge color. The susceptibilities of the isolates against various antibiotics were studied by using two methods; the disc diffusion and broth dilution methods. It was found that isolates were 100 % sensitive to: ampicillin, gentamycin, cloxacillin, lincomycin, rifampicine, cefotaxime, erythromycin, and chloramphenicole. Other isolates were 100 % resistance to: neomycin, colistin, metroinazole, and nalidixic acid, which can be attributed to the exaggerated use in Iraq. The MIC`s of some antibiotics against G. vaginalis were determined, and it was found that there is significant variations in these values compared to that reported in the literature. The resistance of G. vaginalis local isolates was increased on broth growing compared to that on agar, for the following antibiotics: gentamycin, erythromycin, chloramphenicole, streptomycin and co-trimaxazole, and it was explained on the basis of the measurement method accuracy. Mixed ligand complexes of 5,5-diphenyl-imidazolidine-2,4-dione and pyridine, [(C15H12N2O2)2(C5H5N)2M(II)] (M(II) = Cu, Ni, Zn, Cr, Mn, Fe, and Co) were synthesized, and characterized by common physicochemical techniques. Two molecule of pyridine and two molecule of 5,5-Diphenyl-imidazolidine-2,4-dione were coordinated through pyridine nitrogen and deprotonated nitrogen at position-3, respectively. The MIC and MBC of these complexes were determined and it was found that they inhibit the growth of Gardnerella vaginalis at 10-6 M concentration. From the findings of this study, we can conclude that these complexes can be used in topical pharmaceutical formulation to inhibit the growth of G. vaginalis bacteria in case of female bacterial vaginosis.
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