Table of content

Iraqi Journal of Biotechnology

المجلة العراقية للتقانات الحياتية

ISSN: 18154794
Publisher: Baghdad University
Faculty: Institute of genetic Engineering and Techno-biology
Language: English

This journal is Open Access

About

Iraqi Journal of Biotechnology was founded in 2001 ,it was first issued in 2002,it is a semi-annual refereed scientific journal issued by the Institute of Genetic Engineering and Biotechnology in Baghdad University in fields of biology, environment, agricultural sciences ,medicine, dentistry, pharmacology, veterinary medicine and researches specialized in bioinformatics

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E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

Table of content: 2008 volume:7 issue:1

Article
MICROBIAL LIPASES: POTENTIAL BIOCATALYSTS FOR THE FUTURE INDUSTRIES
اللايبيزات المايكروبية: المحفزات الحيوية القادمة للصناعات المستقبلية

Authors: عصام فاضل الجميلي
Pages: 1-20
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Abstract

Today, lipases stand among the most important biocatalyste which carrying out novel reactions in both aqueous and nonaqueous media. This is primarily due to their ability to utilize a wide spectrum of substrates, high stability towards extremes of temperature, pH and organic solvents, and chemo-, region- and enantioselectivity. More recently, the determination of their three-dimensional structure has thrown light into their unique structure-function relationship. Among lipases from plant, animal and microbial origin, it is the microbial lipases that find immense application. This is because microbs can be easily cultivated and their lipases can catalyse a wide variety of hydrolytic and synthetic reactions. Lipases find use in a variety of biotechnological fields such as food and dairy, detergent, pharmaceutical, agrochemical and oleochemical industries. Lipases can be further exploited in many newer areas where can serve as potential biocatalysts.


Article
STATISTICAL AND CYTOGENETIC STUDY ON CHRONIC MYELOID LEUKEMIA PATIENTS IN IRAQ
دراسة احصائية ووراثية خلوية لمرضى ابيضاض الدم النقياني المزمن

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Abstract

This study was carried out on Chronic Myeloid Leukemia(CML) patients, who were attending the National Center of Hematology for the duration between 1/4/1999 to 1/10/2005, to shade light on the distribution of the disease in Iraq beside, studying the chromosomal aberrations in white blood cells of the patients . It includes (537) the total number of CML patients from all over the country, Chi- square test has been used in the statistical study. The ages of the patients arranged (8-75) year old . The results shows that, the highest incidence occurred among group (31-40) and (41-50)blood group(O) was the most affected than the other groups beside that male were more incident than female.


Article
STUDY OF ANTIMICROBIAL ACTIVITY OF SALIX
في Salix acmophylla دراسة الفعالية التثبيطية لمستخلصات نبات الصفصاف

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Abstract

ABSTRACT Antimicrobial activity of cold aquous and ethanol extracts with six concentrations of each one (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %) mg/ml of Salix acmophylla leaves were estimated against four types of pathogenic bacteria (E. coli, S. aureus, Klebsiella spp., St. pyogenes) after incubation at (37)oC for (24)hr. by using well diffuion assay method. Antimicrobial activity of both extracts was compared with that for a number of antibiotics include Ampicilin, Cephalexin, Erythromycin, Amoxicilin, Gentamicin and Tetracyclin, by using antibiograme test. Both extracts of Salix acmophylla showed clear antimicrobial activity against pathogenic bacteria. This activity was enhanced with the increasing of extracts concentrations. The 0.6% concentration of both extracts gave highest activity against E. coli, S. aureus and St. pyogenes with no antimicrobial activity against Klebsiella spp. E. coli was the least testing bacteria affective by the concentrations of both extracts. While S. aureus was the most effective one by the concentrations of aquous extract and St. pyogenes was the most affective one by the concentrations of ethanolic extract. Antimicrobial activity of cold aquous and ethanolic extracts of Salix acmophylla was better than that to Gentamicin against St. pyogenes and S. aureus, while the inhibition effect of Tetracyclin was better than both extracts concentrations against S. aureus and E. coli. The inhibition effects of Gentamicin and Erythromycin and both latest two concentrations of both extracts against E. coli were closely related. Klebsiella spp. was sensitive to Gentamicin only. A resist of pathogenic bacteria to Ampicillin, Amoxicilin and Cephalexin was observed.


Article
STUDY OF ANTIMICROBIAL ACTIVITY OF SALVIA
دراسة الفعالية التثبيطية لمستخلصات نبات المرميةSalvia officinalis في نمو بعض أنواع البكتريا المرضية

Authors: سهاد عدنان أحمد
Pages: 51-63
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Abstract

ABSTRACT Antimicrobial activity of cold aquous and ethanol extracts with four concentrations of each one (0.1, 0.2, 0.3 and 0.4 %) mg/ml of Salvia officinalis leaves were estimated against four types of pathogenic bacteria (E. coli, S. aureus, Klebsiella spp. and St. pyogenes) after incubation at (37)oC for (24)hr by usingWell Diffuion AssayMethod. Antimicrobial activity of both extracts was compared with that for a number of antibiotics include Ampicilin, Cephalexin, Erythromycin, Amoxicilin, Gentamicin and Tetracyclin by using antibiograme test. Both extracts of Salvia officinalis showed clear antimicrobial activity against pathogenic bacteria, which this activity was enhanced with the increasing of concentrations belongs to them. The latest concentration (0.4%)mg/ml of both extracts gave highest activity against E. coli, Klebsiella spp. and St. pyogenes with no antimicrobial activity against S. aureu and E. coli were the least testing bacteria affective by the concentrations of both extracts, while St. pyogenes was the most affective one. Antimicrobial activity of cold aquous and ethanol extracts of Salvia officinalis was better than that to Gentamicin against Klebsiella spp., while the antimicrobial activity of latest concentration of ethanolic extract against St. pyogenes was better than the effect of same antibiotic. The effect of Gentamicin , Erythromycin and Tetracyclin against E. coli was better than that of both extracts concentrations.The concentrations of both extracts had no clear antimicrobial activity against S. aureus ,which affect by Gentamicin and Tetracyclin. A resist of pathogenic bacteria to Ampicillin, Amoxicilin and Cephalexin was noticed.


Article
STUDY THE EFFECT OF LACTOBACILLUS AS

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Abstract

ABSTRACT This study included to investigate the types of Lactobacilli which found in the milk of 35 nursing mothers, and study the effects of these normal friendly bacteria on Candida albicans. Eleven species of the genus Lactobacillus were isolated and distributed as ; thirteen isolate belong to L. plantarum (26 %) , (12) isolate to L. gasseri (24 %) , while L. acidophilus represent (6) isolates (12%) , L. fermentum(5) isolates (10%) , L. casei (3) isolates (6 %) , beside 2 (4 %) isolates for each of L. lactis , L. rafinotactis , L. paracasei , L. rhamnosus , and L. crispatis and only one isolate belongs to L. salivaris (2%) . Candida albicans was diagnosed in 25 samples of saliva from infants affected by thrush. The susptibility of C. albicans was tested against five antifungal drugs The results revealed that the isolates were sensitive to Miconazole , Ketoconazole and Clotrimazol , the zone of inhibition reached to 11, 10 and 10 mm. respectively ; on the contrary, the isolates were resistance to the Nystatin and Grisofulvin . The effect of Lactobacillus spp. filtrate were examined against C. albicans and the results were clarified that there was inhibition activity of L. acidophilus and L. fermentum against it, with zone of inhibition reached to (28), (15) mm. respectively. When the effect of Lactobacillus spp. extract on C. albicans DNA was measured , by spectrophotometer at different wave lengths ( 260, 280, 361 nm ) respectively they showed increase of the absorbance with increasing of the amount of extract , which would mean that the extract cause distraction to the DNA of the C. albicans .


Article
STUDY OF INHIBITORY ACTIVITY OF VOLATILE OIL STUDY OF INHIBITORY ACTIVITY OF VOLATILE OIL
دراسة الفعالية التثبيطية للزيت الطيار ومطحون أزهار نبات البابونج في بعض الأحياء المجهرية المرضية

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Abstract

ABSTRACT Volatile oil was extract from Matricaria chamomilla and Anthemas nobilisd flowers using clavenger devise which was 0.3% and 0.7% for first and second type respectively. The inhibition activity of volatile oil was studied using agar well diffusion method on some pathogenic microorganisms like Escherichia coli, Staphylococcus aureus and Candida albicans and the result showed that the volatile oil had an inhibitory effect on the growth of these microorganisms. The minimum inhibitory concentration (MIC) value for E. coli and St. aureus was (2.5)%and(0.625)% respectively and (2.5)% for C. albicans.while the minimum bactericidal concentration (MBC) for E.coli was (5)% Staph.aureus was(1.25)% and C.albicans was(5)%. The chamomile flower powder also showed inhibition activity for these microorganisms and the (MIC) was (0.125),(0.0312),(0.125)gm/ml for E. coli, St .aureus and C. albicans respectively, while the (MBC)was (0.250),(0.061),(0.250)gm/ml ,respectively . It was accomplished in this study also formulation of ointment for external use contain 5% (v/v) volatile oil and ointment contain 20%(w/w) chamomile flowers powder and two formula were evaluated using High Performance Liquid Chromatography (HPLC).


Article
التأثير السمي الوراثي لمبيد القوارض (البروديفاكوم) في الخلايا المولدة للنطف والخلايا الامية للبيوض في الفئران

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Abstract

ABSTRACT In this study three doses of rodenticide brodifacoum have been investigated for the frequency of chromosomal aberrations in metaphase 1 chromosomes recovered from oocytes was compared with those recoverd from spermatocytes in pachytene and diplotene phases. The results showed no affect appears in oocytes and spermatocytes after one day, but it was effected in 7, 21 , 35 day after treatment. These doses induced singnificantly higher chromosomal aberration specially after 21 and 35 day.The frequency of aberration in oocytes did not differ in spermatocytes during diplotene, but significantly more chromosome aberrations were found in pachyten phase compared with those in diplotene. The pachytene is more sensitive to rodenticide.Increased chromosomal aberration especially after 21, 35 day.Conclusion of these tests were revealed that the brodifacoum had a significant cytogenetic and genotoxic effects. These effects were more in spermatocytes than oocytes.

Keywords

Rodenticide --- brodifacoum --- genotoxic --- mice.


Article
EFFICENCY OF LOCAL ISOLATES BACTERIA EFFICENCY OF LOCAL ISOLATES BACTERIA
كفاءة سلالتي البكتريا المحلية Pseudomonas aeruginosa في معالجة مياه المخلفات النفطية Serratia ficaria

Authors: أمل علي حسين
Pages: 96-109
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Abstract

الخلاصة تم أختبار قابلية نوعين من البكتريا المحلية في معالجة مياه المخلفات النفطية المطروحة من مصفى الدورة الواقع عند الجزء الجنوبي من مدينة بغداد على الضفة اليمنى من نهر دجلة، إذ تم معالجة المخلفات النفطية باستخدام تقنية المزارع الخليطة بإضافة العزلتين API المتجمعة في الحوض المسمى وأعتمد على أكثر من مؤشر Serratia ficaria (AA و ( 39 Pseudomonas aeruginosa (AA22) لتعيين ومعرفة كفاءة عملية المعالجة ومقارنة هذه المؤشرات مع نظيرتها في المياه المعالجة بالكائنات المجهرية المستخدمة في المصفى (اللقاح التجاري المكون من الابتدائيات). وجد أن الخليط المكون من عزلتي البكتيريا هو الأكفأ في معالجة مياه المخلفات (في ظروف التجربة) والتخلص من أكبر قدر من المواد الصلبة والهيدروكاربونات، إذ انخفض المتطلب الحيوي والكيميائي للاوكسجين من 7.27 ملغم/ لتر و 800 ملغم/ لتر الى 3.73 ملغم/ لتر و 600 ملغم/ لتر على التوالي و للقاح التجاري 175 ملغم/ لتر و 849 ملغم/ لتر بعد ان كانت 461 ملغم/ لتر و 868 ملغم/ لتر على التوالي.


Article
THE ACTION OF IRAQI PROPOLIS ON THESPECIFIC ACTIVITY OF ADENOSINE DEAMINASEENZYME IN ALBINO MICE
تأثيرالعكبر العراقي في الفعالية النوعية لانزيم الادينوسين دي امينيزفي الفئران البيض

Authors: غسان محمد سليمان
Pages: 110-116
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Abstract

ABSTRACT
The action of propolis in the specific activity of adenosine deaminase (ADA; EC
3.5.4.4) in albino mice was investigated. Two oral administrations (10 and 20
mg/kg/day) of Iraqi propolis extract with two control groups were used. Treatment
with propolis enhanced the specific activity of ADA enzyme in serum, thymus and
spleen as compared to control groups; the stimulation was greater at the higher
dose. While, treatment with Mitomycin C; MMC (positive control) under similar
condition caused a highly significant inhibition in activity of ADA in serum,
thymus and spleen as compared to negative control. This study indicates that Iraqi
propolis extract may potentiate immunity with the modulation of adenosinergic
system through increasing splenic and thymic ADA enzyme activity.
KeyWords: Propolis, adenosine deaminase, mitomycinC

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Article
GENETIC MUTATION OF PSEUDOMONASAERUGINOSA OM13 TO IMPROVE ITS PRODUCTIONOF ANTIFUNGAL COMPOUND
Pseudomonas aeruginosa OM التطفير الوراثي لعزلة البكتريا 13لتحسينانتاجها من المركب المضاد للفطريات

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Abstract

ABSTRACT
The ability of P. aeruginosa OM13 in antagonizing or inhibiting the growth of
some phytopathogenic fungi was tested by measuring the inhibition zone for the
growth of the tested fungi (Alternaria alternata and Rhizoctonia solani) caused by
the growth of the bacterial isolate (P. aeruginosa OM13 ) and by the action of the
culture filtrate of this isolate on test fungi. It was found that this isolate inhibit the
growth of the test fungi and the inhibition zones were 5mm, 7mm for Alternaria
alternata and Rhizoctonia solani, respectively. To improve the antifungal
compound production by P. aeruginosa OM13, it was treated with mutagenic agent
nitrosoguanidine (MNNG). The results showed that the production of 5 isolates
were highly increased, in which RA5 gave the highest inhibition zone and O.D520nm
values when compared with other. An attempt to characterize the partially
purified antifungal compound produced by P. aeruginosa OM13 by using infrared
(IR), UV absorbance and Thin Layer Chromatography (TLC) were done and the
results showed that the compound appeared as a single dark spot on a TLC plate
under UV-light that had Rf value 0.32 from IR, UV analysis. It was confirmed that
the compound was aromatic and contain aliphatic group. It can be concluded that
the active antifungal compound might be either pyocyanine which is a derivative of
the antibiotic phenazine or might be a new derivative of phenazine.
Key words: Genetic mutation, Pseudomonas aeruginosa, production, antifungal

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