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Iraqi Journal of Biotechnology

المجلة العراقية للتقانات الحياتية

ISSN: 18154794
Publisher: Baghdad University
Faculty: Institute of genetic Engineering and Techno-biology
Language: English

This journal is Open Access

About

Iraqi Journal of Biotechnology was founded in 2001 ,it was first issued in 2002,it is a semi-annual refereed scientific journal issued by the Institute of Genetic Engineering and Biotechnology in Baghdad University in fields of biology, environment, agricultural sciences ,medicine, dentistry, pharmacology, veterinary medicine and researches specialized in bioinformatics

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E-mail:journal@ige.uobaghdad.edu.iq
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Table of content: 2006 volume:5 issue:1

Article
IMPROVEMENT OF MEAT QUALITY CHARACTERISTICS OF AGED FEMALE GOATS BY ELECTRICAL STIMULATION AND SALT SOLUTIONS
تحسين بعض الصفات النوعية للحوم ذبائح إناث الماعز المسنة باستعمال التحفيز الكهربائي والمحاليل الملحية

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Abstract

The objective of present study was to investigate the effect of electrical stimulation and salt infusion on improvement of some meat quality characteristics of aged female goat. Thirty female goats, age 7-8 years and 40.50 kg in weight were managed, fed a like and slaughtered thereafter. The carcasses were divided into 7 groups and each group were subjected to one of the following treatments : T1 : Control , T2 : electrical stimulation (80 volts , 25 Hz for 2 min), T3 : electrical stimulation (200 volts , 25 Hz for 2 min) , T4 : sodium chloride infusion (0.6) , T5 : calcium chloride infusion (0.3 M) , T6 : T2 + T4 and T7 : T2 + T5. Results obtained may be summarized as follows: The pH values in Longissimus dorsi (LD), Semimembranosus (SM) and Bicepe Fomoris (BF) in T2 and T3 declined rapidly and the muscles required the shortest period of time to reach pH 6 in comparison with the other treatments. There were no significant differences among the treatments in temperature decline, drip loss, thaw loss and cooking loss. Higher water holding capacity values were obtained with LD in T7 (30.47%), SM in T5 (28.10%) and BF in T5 (30.25%) whereas, the lowest values for the three muscles were in T1, LD also surpassed the other two muscles in water holding capacity. There were significant differences among the treatments in sarcomere length, where BF was superior to other muscles. It can be concluded from this study that electrical stimulation and infusion of NaCl or CaCl2 improve meat quality characteristics of aged female goat meat. ___________


Article
GENETICAL, CYTOLOGICAL & BIOLOGICAL STUDIES OF OWSWF, CHRYSOMYA BEZZIANA EXPOSED TO GAMMA RAYS: VIII. KARYOTYPE OF FIELD & LABORATORY STRAINSI
دراسات وراثية وخلوية وحياتية لذبابة الدودة الحلزونية للعالم القديم المعرضة لأشعة كاما: طبيعة النواة للسلالتين المختبرية والحقلية .VII

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Abstract

The karyotype of field & laboratory strains of OWSWF, Chrysomya bezziana was investigated. The results of this investigation showed that this species has six pairs of chromosomes (2n = 12), which consist of five pairs of autosomal chromosomes and a pair of sex chromosome. The average length of autosomal chromosomes of the field & laboratory strains were 4.03, 3.77, 3.46, 3.05, 2.41, &4.00, 3.68, 3.38, 2.98, 2.53 mmfor chromosomes numbers 2, 3, 4, 5& 6 respectively, while for the sex chromosome, it was difficult to be measured because they form what is like parachute shape. Furthermore, in this investigation the arm & centromere indeces were also measured for field & laboratory strains. Lastly , the results of different mating between these two strains of Ch. bezziana showed that they were belong to species Ch. bezziana.


Article
EVALUATION OF POLYSACCHARIDES PRODUCED BY PSEUDOMONAS B-7741 USING THIN LAYER CHROMATOGRAPHY TLC
تقييم متعدد السكريات المنتجة من قبل البكتريا 7741 - Pseudomonas Bكروموتوغرافيا الطبقة الرقيقةTLC

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Abstract

The study has tackled about the increase of Pseudomonas B-7741 recently diagnosed and fed by methanol in modified M9 medium. The polysaccharides have been extracted from medium by precipitating them using some organic solvents and the precipitant is isolated and dried. After the deconstruction process of polysaccharides to monosaccharides, the constituents of polysaccharides are isolated by using thin layer chromatography TLC. The study has shown that polysaccharides produced by Pseudomonas B-7741 bacteria consists of D- Glucose and D-Galactose. ____________________________________________________________________


Article
THE APPLICATION OF SOME AGAR ALTERNATIVES IN MICROPROPAGATION OF THREE GRAPE(Vitis vinifera L.) VARIETIES
استعمال بعض المواد المصلبة للوسط الغذائي بدلاً عن مادة الآكار في اكثار ثلاثة أصناف خارج الجسم الحي Vitis vinifera L. من العنب

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Abstract

Some agar alternatives were used for solidifying the half strength MS medium using corn starch, potato starch, ice cream thickener (Thalabia) and a mixture of potato and gelatin. Those agents were tested for the micropropagation of three European grape varieties Vitis vinifera L. namely, Ollivate blane (Fransi Aswad), Halawani and Kamali. Results showed that using corn starch or potato starch instead of agar inconsistent in solidifying the culture media. Meanwhile, mixing potato starch with gelatin or ice cream thickner individually proved promising solidifying agents. The addition of 1 mg/ 1BA to the culture media increased the number and length of shoots significantly. No significant differences appeared between such gelling agent used. The three grape varieties produced roots characterized with its length and leaser branching when cultures on auxin free medium compared with those grown on auxin containing media. It is concluded that the three varieties of grape can be micropropagated on some cheap agar alternatives instead of the costly agar. ____________________________________________________________________


Article
استخدام تقنية الاليزا والالفا لتشخيص الاصابة بفايروس التهاب الكبد نوع B و (HIV) وفايروس الايدز عند مرضى الهيموفيليا العراقيينC

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Abstract

This study was carried out on haemophilic patients (type A and B) from the 1st of October 1997 to the 1st of October 2004 on patients attended the department of Inherited Hemorrhagic Diseases, Al-Mansour Hospital / Baghdad. 100 blood samples were collected from haemophilic patients for laboratory examination in the AIDS Research and studies centre /Baghdad. By using different serological techniques like ELISA , ELFA and confirmatory tests . The result revealed the presence of 474 haemophilic patients. Haemophilic patients type A was 77.8%, while type B was 22.2%. Chi-sequare analysis indicated clear significant (p<0.001) relationship of haemophilic patients type A and B blood groups. The results also indicated the presence of hepatitis C virus (25%),while hepatitis B virus was (3%) and HIV was 6%). ____________________________________________________________________

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Article
ULTRASTRUCTURAL AND GENETIC ANALYSIS OF MUCOGENICITY IN KLEBSIELLA PNEUMONIAE
تحليل تركيبي ووراثي للزوجة بكتريا الكلبسيلا الرئوية

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Abstract

A mucoviscous clinical respiratory isolate of K. pneumoniae was found to produce 414 ng/106 CFU of polysaccharide. Transmission Electron Microscopy (TEM) revealed that the isolate has a dense thick capsule with a diameter of 190 nm. The isolate was harboring a single large plasmid encoding multiple resistance to antibiotics. The isolate was subjected to successive subculturing for 480 generation on M9 medium with and without 150 μg/ml salicylic acid at 37 ºC. Six mucoid variants were obtained, five (A, B, C, D and E variants) after salicylic acid treatment. The six derivatives varied in the quantity of polysaccharides produced, genetic and physical behavior and their fine structures as indicated by TEM photography. One of the variants (variant A) has a small capsule and its colonies lost the mucoviscous phenotype. Another (variant B) a non-capsulated as indicated by fluorescent antibody staining with antigen serum of wild type. Plasmid DNA was extracted from each of the six Klebsiella variants and transformed into E.coli MM294 competent cells. The transformed E.coli showed variable mucoid traits and resistant profile with temperature dependent gene expression of mucogenicity; one of the variants derived plasmid (pKP10) induced mucoid phenotype in E.coli at 37ºC, altered cell division at 37ºC and lead to its inability to ferment lactose on MacConkey medium at both 30 and 37 ºC.


Article
EFFECT OF SUB INHIBITORY CONCENTRATIONS OF ANTIBIOTICS ON ADHERANCE OF PSEUDOMONAS AERUGINOSA
تأثير التراكيز تحت الدنيا من بعض المضادات الحياتية على قابلية التصاق بكتريا Pseudomonas aeruginosa

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Abstract

ABSTRACT Susceptibility of various isolates of P. aeruginosa to various antibiotics was tested. Ciprofloxacin was found to be the most effective antibiotic. Minimum inhibitory concentrations for ciprofloxacin, Gentamicin, piperacillin and cefotaxime, were specified and ciprofloxacin was found having the lowest MIC as compared with others . The effect of sub MIC ( ¾ , ½ , ⅓ and ¼ ) of these four antibiotics on adhesion capability of the isolates was tested. Cefotaxime was uneffective as comparied to gentamycin, ciprofloxacin and piperacillin. ¾ MIC of ciprofloxacin was found the most effective concentration for attenuation of adherence capability. __________________________________________________________


Article
PREPARATION OF C-REACTIVE PROTEIN LATEX REAGENT OF HUMAN SERUM
تحضير محلول لاتكس لبروتين Cالمنفعل من مصل الإنسان

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Abstract

ABSTRACT C-reactive protein (CRP) is an acute phase reactant the concentration of which increases significantly following tissue injury or inflammation. It is present in the albumin fraction of the serum of patients and is antigenic upon injection into rabbits. We report here the development of a local latex reagent coated with rabbit anti-CRP. For this purpose CRP was isolated from pooled sera of patients and injected into rabbits. CRP rabbit antiserum was purified by using ammonium sulfate precipitation. Followed by passing on ion exchange chromatography (Diethylaminoethyl "DEAE" cellulose). Then, latex particles were coated with purified antisera. The prepared CRP latex reagent agglutination was observed upon the addition of patients' sera and compared with that of normal sera. In addition, it was evaluated against a standard CRP latex reagent. The prepared reagent gave positive results (100%) with patients' sera and the positive control of the standard kit. While, the negative control of the standard kit and 30% of the normal sera gave negative results.


Article
EFFECT OF PLASMID SIZE ON THE TRANSFORMATION OF E.COLI HB101
تأثير حجم البلازميد على تحول بكتريا القولون E.coli HB101

Authors: Abdul Hussain Moyet Al-Faisal
Pages: 123-131
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Abstract

Plasmids are one of the most common vectors used in cloning. Many factors have been repotted to affect the bacterial transformation by plasmids. This paper an attempt to find the effect of plasmid size on the transformation efficiency of E.coli HB101. Our results showed that the small sized Plasmids more effective in transforming of the bacteria E.coli HBCOL was) (4.1-4.8kb than the large ones (5.7-6kb). The transformation efficiency with the small sized plasmids reaches between 6×610-7×610 comparing with 4.1 ×105- 4.6×105 of the large sized plasmid. We conclude that the small sized plasmids are more suitable to use in cloning than large sized plasmids.

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