Table of content

Iraqi Journal of Biotechnology

المجلة العراقية للتقانات الحياتية

ISSN: 18154794
Publisher: Baghdad University
Faculty: Institute of genetic Engineering and Techno-biology
Language: English

This journal is Open Access

About

Iraqi Journal of Biotechnology was founded in 2001 ,it was first issued in 2002,it is a semi-annual refereed scientific journal issued by the Institute of Genetic Engineering and Biotechnology in Baghdad University in fields of biology, environment, agricultural sciences ,medicine, dentistry, pharmacology, veterinary medicine and researches specialized in bioinformatics

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E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

Table of content: 2011 volume:10 issue:2

Article
ENUCLEATION... THE STARTING POINT FOR BIOLOGICAL CLONING
إزالة (فصـع) الأنوية ... نقطـة البدايـة للإستنسـال البـايـولـوجـي

Authors: أياد محمد علي فاضل
Pages: 149-167
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Abstract

The Enucleation Technique considered as the first and most important step to accomplishe any biological cloning process which includes also Fusion; Activation ,and Embryo transfer. This Review Article was carried out to elucidate, the different Enucleation methods since the first attempt of “Brigg”and “King” (1952) and by others till (2011) including the physical methods such as UV Irradiation to Enucleate the Amphibian nuclei or using Microsurgery and/or both Techniques. The Microsurgery technique includes two methods: the Enucleation of nuclei of the MII Oocyte and the Enucleation of nuclei of the Telophase II Oocyte. The review article includes also the Enucleation using the chemical methods which includes the cytochalsin B; Enucleation using Hypertonic solution; and Enucleation using Demecolcine and Nocadazole.


Article
THE OPTIMUM CONDITIONS FOR ENDOGLUCANASE PRODUCTION FROM ASPERGILLUS FLAVUA
دراسة الظروف المثلى لانتاج Endoglucanaseمن العزلة المحلية لفطر Aspergillus flavus

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Abstract

ABSTRACT Fifteen local fungal isolates produced endoglucanase (EC. 3.2.1.4) from different areas belonged to five genera namely Eggplant, corn, millet, tomato, Wheat and soils were subjected to endoglucanase production test, the isolate Aspergillus flavua was selected based on its high production endoglucanase was reach 1 unit/ml. The optimal condition for production endoglucanase using modify czapekdox contained 2% carboxyl methylcellulase, 0.2% ( NH4)2 HPO4, 0.5% MgSO4 2H2O, 1% K2HPO4, 0.5% KCL, 0.01%FeSO4 2H2O and optimum inoculia level was 106 spore /ml and the initial PH was 5 after 8 day incubation period was at 30°C and the production was reach 4.95 unit/ml.


Article
PREDICTION OFALLERGENICITY OF CRY PROTEINS PRODUCED BY BACILLUS THURINGIENSIS USINGBIOINFORMATIC TOOLS
التنبؤ بقابلیة تولید الحساسیة لسموم Cry Bacillus المنتجة من البكتریا thuringiensis بإستعمال وسائل المعلوماتیة الحیویة

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Abstract

ABSTRACT Bioinformatics performs a lot of tools for biological studies, among those prediction of allergenicity. The study carried out to predict allergenicity of Cry toxins produced by Bacillus thuringiensis which their genes are used in production of plants resist to insects. Five hundreds and eleven Cry protein sequences were downloaded from NCBI. The proteins subjected to investigation of their composition of amino acids and molecular weights, estimation of pI and net charge, pepsin digestion at pH 1.2 and >2.0 and estimation of hydrophobicity. Results revealed that proteins are greatly different in their number of amino acids which ranged from 78-1825 residues and MW 8587.42-251932.0 Dalton. The pI was 4.36-10.16 and was proportional to the net charge of proteins. Using AllermatchTM program recommended by FAO/WHO, output pointed to similarity of 0.78%(4 out of 513) of protein to those of IgE epitopes of documented allergens. Similarity >35% over 80 amino acids window revealed that 3.72% (19 out of 513) of Cry proteins similar to known allergens. Using Allergen online website which is slightly different from AllermatchTM showed no similarity with allergens (included in its databases). Using specialized program for IgE epitopes i.e; AlgPred revealed no similarity as well


Article
EFFECT OF HG, CU, NI MINERALS' ON BIOFILMEFFECT OF HG, CU, NI MINERALS' ON BIOFILM
تأثیر معادن الزئبق والنحاس والنیكل في تكوین الغشاء الحیوي لبكتریا لPSEUDOMONAS AERUGINOSAلعناصر الثقیلة

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Abstract

The present study is conducted to in restigate the bacteria Pseudomonas to the heavy metals. The isolates themselves are submitted to a test to examine their resistance to three types of minerals (cooper, mercury and nickel ) and their impact on the growth of the isolates and the formation of biofilms. Besides, the ability of these isolates in the treatment of heavy metals and removing them from the environment. The results of using the heavy materials indicate that mercury is most effective when it is inhabited for the purpose of the growth of local isolates at a concentration 12.5 mg.l-1compared to nickel and copper which inhibit the growth of all isolates at concentration 300 mg.l-1. While it is found that nickel has a vitas effect on the formation of biofilms of all the isolates where one can find that the formation of membrance is stopped to all isolates at concentration 200 mg.l-1. Actually, the biofilms is formed at a concentration between (0.25–10)mg.l-1under the effect of mercury and then it is stopped. At all at concentration 250 mg/l. In relation to the copper one can find that the formation of biofilms is continued until it reaches to the concentration 250 mg.l-1 and its formation is stopped at all at concentration 300 mg.l-1 The effeciency of the five local isolates is varied in removing the heavy minerals(nickel, copper, mercury) from the cultural media .The results show the ability of these isolates in removing these minerals with a high efficiency of concentration (5–250) mg.l-1after that the efficiency of these isolates is decreased in removing these minerals with the increase of concentration of the mineral in cultural media


Article
STUDY THE ANTIMICROBIAL ACTIVITY OF PROANTHOCYANIDIN (POCS) FROM GRAPE SEEDS
دراسة الفعالیة التثبیطیة لمُركب البروانثوسیانیدین المُستخلصمن بذور ثمار العنب على بعض الأحیاء المجھریة

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Abstract

ABSTRACT Proanthocyanidin (POCs) was extracted from grape seeds by liquid-liquid chromatography and identified via high performance liquid chromatography (HPLC).The antimicrobial activity of POCs was determined against nine of gram positive and gram negative bacterial strains and Candida albicans by agar well diffusion method and two strains of dermatophyte fungi Trichophyton interdigital and Trichophton menagraphytes. It was found that POCs has inhibition effect against Staphylococcus aureus, Streptococcus pyogenes and Bacillus subtilitis which gave inhibition zone (16, 14.5, 11) mm/ml respectively at the concentration 100mg /ml While there was low no inhibition effect against gram negative bacteria and Candida albicans, On the other hand the inhibition percentage against dermatophyte fungi T. interdigital and T.mentagraphytes increased with increasing the concentration of POCs which reached for 64.7%and 44.4%respectively


Article
DETERMINATION OF PLASMIDS ROLE IN BACTERIOCIN PRODUCTION FROM PATHOGENICKLEBSIELLA
تحدید دور البلازمیدات في إنتاج البكتریوسین من الكلبسیلا المرضیة

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Abstract

One hundred and twenty five sample were collected as(50 Burne+wound and75 blood samples)from hospital patients (Ibn Albalady and Alkindy Hospital in Baghdad at period Nov .2010. to March. 2011). 40 bacterial isolates were isolated of which 18 isolates showed similar characteristic to Klebsiella depending on phenotypic characterization and biochemical test and using with AP1 20E system and VITEK 2. Agar disc were used to screen the isolates for their ability of bacteriocin production. The result showed that7isolates had the ability of bacteriocin production specially Klebsiella terrigena that show the highest production. Plasmid content was studied for Klebsiella isolates .Results showed that some isolates has plasmid and genomic DNA. To determine the role of plasmid in bacteriocin production, two highest isolates were selected (Klebsiella terrigena 7 and Klebsiella terrigena 6 )to perform plasmid curing experiment using Ethidium bromide. Result showed that the plasmid has been cured at 512 mg /ml Ethidium bromide. the cured cells lost their ability of bacteriocin production which proved the bacteriocin gene is located on plasmid. To confirm this result, transformation experiment was performed by transferring the plasmid the extracted from Klebsiella terrigena 7 to the standard strain E.coli MM 294rif + which is free of plasmid and have no resistance to Ampicillin (Amp), the result showed that, the E.coli MM 294rif + strain become Amp resistant in addition it had the ability to produce the bacteriocin.

Keywords

Klebsiella --- Bacteriocin


Article
PROGRAMMED CELL DEATH IN PATIENTS WITH
الموت المبرمج للخلایا في مرضى سرطان القولون

Authors: أریج حمد جعفر
Pages: 242-249
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Abstract

ABSTRACT In this study forty cases were investigated for evaluation of apoptosis in patients with colon cancer by using the TUNEL assay. The results showed that the number of TUNEL positive colonic epithelial cells were significantly different from that in the control group and the frequencies and percentages of apoptosis between patients and controls were statistically significant (p < 0.05)

Keywords

Colon Cancer --- Apoptosis.


Article
ROLE OF ZEBRA PROTEIN OF EPSTEIN-BARR VIRUS
دور بروتین ZEBRAللفایروس ابشتاین بار EBV وتأثیره على تعبیر بروتین p السیطرة 53في مرضى الهوجكن واللاهوجكن لیمفوما

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ABSTRACT Hodgkin's lymphoma ( HL) is uncommon malignant tumor of the lymphatic system, some were shown to contain clonal, Epstein-Barr virus (EBV). Non Hodgkins lymphoma (NHL) are associated with EBV, ZEBRA protein plays a dominant role in the switch from latent cycle to productive infection of the virus. To access the expression of ZEBRA protein in Hodgkin's and non Hodgkin's lymphoma patients and its impact on P53 expression. A total of 28 of patients newly diagnosed to have HL and NHL investigated for EBV ZEBRA protein and p53 expression by immunohistochemistry technique. p53 was expressed in 80.9% and 100% of males and females respectively. In this study ( 86.7%) of HL and 88.6%of NHL cases were p53 positive. 87.5% of mixed cellularity were p53 positive. While 83.3% intermediate grade were p53 positive .No significant difference found between p53 expression and gender, type of lymphoma but there is significant difference with histopathological type. In regard to ZEBRA protein ,it is found that all the males and females with HL and NHL cases under study showed ZEBRA positivity.Also the 16 cases of the mixed cellularity type and all the 12 cases of intermediate grade are ZEBRA positive. No significant difference found between ZEBRA expression and gender, but there is significant difference with type of lymphoma and the histopathological type. This study shows that there is interaction between ZEBRA and p53 which depend on the relative amounts of those two proteins as confirmed previously and the expression of ZEBRA protein in lymphoma reflects the immunocomprimized state of patients and confirmed that EBV contribute to the development of malignancy. Key words : Hodgkin's lymphoma, Non Hodgkin's lymphoma, p53 tumor suppressor, ZEBRA protein.


Article
EXTRACTION AND PARTIAL PURIFICATION OF PROTEASE FROM LOCAL SERRATIA MARCESCENS
الإستخلاص والتنقیة الجزئیة لأنزیم البروتیز من العزلة المحلیة

Pages: 261-272
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Abstract

ABSTRACT Serratia marcescens isolated from a patient with urinary tract infection taken from the Central Health Laboratory. The sensitivity test showed the resistant to the Tetracyclin (30μg), Amoxicillin (25 μg), Gentamycin(30 μg), ampicillin (10μg), and Kanamycin (30μg), but it was sensitive to Ciprofloxacin (5 μg) only. The extracellular protease was extracted in LB broth and purified in two step included precipitation with (30-55%) saturation of ammonium sulfate, dialysis and ion exchange chromatography by DEAE–Cellulose. Only one peak contained the enzymatic activity, and purification fold was 22.6 with 37.92% recovery. Based on the inhibitory effects of EDTA, the protease was characterized as a metalloproteinase. The enzyme activity was increased in the presence of Mg2+ and decreased in the presence of Cu2+ and Zn2+. The protease could also be activated by the non-ionic surfactants Tween 80 (1.0%) and Triton X-100 (1.0%).


Article
DESIGN A FINGERPRINT DATABASE PATTERN RECOGNITION SYSTEM VIA CLUSTER ANALYSIS METHODI- DESIGN OF MATHEMATICAL MODEL
تصمیم قاعدة البیانات كنظام لتمییز أنماط بصمات الأصابع بإستعمال طریقة التحلیل العنقودي

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Abstract

Cluster Analysis Method – Hierarchical Clustering procedure (HCP) was used to classify the fingerprint database into groups. The goal of this paper was to reduce the time that was required to identify the desired fingerprint image of specific person. The results show that the proposed fingerprint database system reduce time that is required to identify the desired fingerprint image in the case of clustering task compared with classical method.

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