Table of content

Iraqi Journal of Biotechnology

المجلة العراقية للتقانات الحياتية

ISSN: 18154794
Publisher: Baghdad University
Faculty: Institute of genetic Engineering and Techno-biology
Language: English

This journal is Open Access

About

Iraqi Journal of Biotechnology was founded in 2001 ,it was first issued in 2002,it is a semi-annual refereed scientific journal issued by the Institute of Genetic Engineering and Biotechnology in Baghdad University in fields of biology, environment, agricultural sciences ,medicine, dentistry, pharmacology, veterinary medicine and researches specialized in bioinformatics

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E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

Table of content: 2013 volume:12 issue:1

Article
Balance of Vaginal System
توازن نظام المهبل

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Abstract

In general the success of biosystem depends on its flora . Human represents superorganism as it contains variable microbial flora which called human microbiome. The latter divided into core microbiome and variable microbiome . Distribution of human microbiome concentrated in systems and organs exposed to the outer environments, such as ; skin, mouth , nose, digestive channel , urogenital channel .Vagina represents one of the secondary environment in the body responsible for reproductive health . and since it exposes to the external environment it should have some sorts of defenses . The components of vaginal defenses are: vaginal epithelial cells and its associates which excretes some defensins. Vagina has its own innate immunity, presence of IgG, IgA. In the vagina, there is some enzymes or enzyme inhibitors such as Stable serine protease inhibitor (SLPI) . In addition there are some more defense facilities such as Mannose binding lectins (MBL) , Chaperone (HSPs) which increased upon infections and inflammations , antimicrobial peptides. The main natural flora of the vagina is Lactobacilli, which classified into Lactobacillus acidophilus complex using routine rout and can be differentiated more using molecular route . These bacteria are responsible for setting the acidic reaction of the vagina . It can protects vagina against different infections and can be used to restore the unbalanced vaginal conditions if it selected carefully according to certain criteria .


Article
Determination the Susceptibility of Bulinus truncatus to the Leaves and Steam Crude Aqueous Solution of Citrus aurantium L.
تحديد حساسية قوقع Bulinus truncatus للمحلول المائي الخام لأوراق وسيقان نبات النارنج.Citrus aurantium L

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Abstract

: This study is a contribution to determine the susceptibility of the snail Bulinus truncatus the intermediate host of urinary Schistosomiasis in Iraq to the crude aqueous solution of Citrus aurantium L. leaves and stems. It was found that the lethal concentration was (4 mg ml)of the crude solution of leaves in the first day of the treatment and was (2.5mg ml) and (4mg ml) of 10 gl and 20gl of the crude solution of stems respectively in the first day of the treatment.


Article
Genetical Effect of Flavonoids Extracted from the Leaves of Camellia sinensis on Mice
التأثير الوراثي للفلافونويدات المستخلصة من أوراق الشاي الأسود في الفئران البيضاء

Authors: Amina S. Jalal
Pages: 30-35
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Abstract

This study was in AL-Nahrain Research Center/University of AL-Nahrain in 2009, examine the role of flavonoids extracted from Camellia sinensis leaves in the inhibition effects of genotoxicity of the chemical Methotrexate (MTX)in mice and depended on some genetic analyzes, which includes the Mitotic index, Chromosomal aberration assay and Micronucleus test in the bone marrow of the mice. This study showed that methotrexate (MTX) agent had toxic effect on cytogenetic by decreasing the mitotic index and increasing the chromosomal aberrations and micronucleus in the bone marrow while the flavonoids extract showed anti-mutagenic activity against methotrexate (MTX) by increasing the mitotic index and decreasing the chromosomal aberrations and micronucleus in the bone marrow.


Article
Effect of Olive Oil Extract on some Biochemical Parameters in Alloxan Induced Diabetic Mice
تأثير مستخلص زيت الزيتون على بعض المعايير الكيموحيوية في الفئران المستحث فيها داء السكري بفعل الألوكسان

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Abstract

Olive oil extract was studied for diabetes mellitus that induced by alloxan in mice to development herbal medicine against the non communicable disease. We have measured the fasting blood glucose level (serum). Hyperglycemia and hyperlipidemia are two important characters of diabetes mellitus. Hyperlipidemia developed in these experimental diabetic mice was assessed by quantification of total cholesterol (TC), high-density lipoprotein cholesterol (HDLc), and triglyceride (TG) in serum, so the extract of olive oil have some remedial effects against alloxan-induced diabetic mice. Forty five adult male mice used in this study, animals were divided into three equal groups :Control without any treatment (A), alloxan treated (B) and group(C) induced diabetic and treated with the extract of olive oil (0.5ml/100g B.W). All animals were sacrificed 2,4,6 weeks post treatment respectively. After treatment of extract of olive oil in alloxan-induced diabetic mice the result showed a significant remedial effect on blood glucose level, None of the extract has any metabolic toxicity induction in general (by measuring level of GPT, GOT and ALP).

Keywords

Olive oil --- alloxan --- hypolipidemic


Article
Detection of Exo A and OPR I Genes in Pseudomonas aeruginosa Using Polymerase Chain Reaction
تحديد جينات exo A و oprI في aeruginosa Pseudomonas بأستعمال التفاعل السلسلي للبوليمريز

Authors: Sana'a N. Hussein
Pages: 44-50
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Abstract

In this study, two polymerase chain reaction (PCR) assays were performed individually for the molecular detection of Pseudomonas aeruginosa obtained from wound and burn samples. Two gene were targeted as specific markers for P. aeruginosa namely the exotoxin A (exoA) gene and the outer membrane lipoprotein (oprI) gene. Seven isolates for each of P. aeruginosa and other Pseudomonas species were employed in this study and boiled bacterial suspension was used as a DNA template. Results showed that all P. aeruginosa isolates gave positive PCR product for oprI gene with amplified size (196 bp) while only 4isolates were harboring exo A gene with amplified size (396 bp). Results for PCR product for Pseudomonas sp. Revealed that none of the seven isolates were positive for exo A gene and only 4 were positive for opr I gene. This research may indicates that molecular detection of P. aeruginosa from wound and burn samples employing exo A and opr I genes by using PCR technique is a rapid method.


Article
Apoptotic Features in Normal Rabbit’s Adenohypophysis Cells (Electron Microscopic and Quantitative Study)
أشكال الذوي في خلايا النخامية الغدية للأرنب(دراسة بالمجهر الإلكتروني ودراسة كمية)

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Abstract

The earliest notable signs of the apoptosis such as the chromatin mass condensation and gradually migration peripherally toward the nuclear envelope were recognized. The terminal end of the apoptotic pathway, represented with apoptotic bodies and membrane blebs or blisters appearances recognized too. In general, apoptotic cells were decreased with progressing animal age; however, there was only significant increase of the apoptotic cells between baby group and adult group in rabbit adenohypothysis with t-value 2.46 and p< 0.05.


Article
Effect of camellia sinensis Methanol extract on Human Lymphocytes in Vitro
تأثير المستخلصات الميثانولية للشاي الاخضر (Camellia sinensis) على الخلايا اللمفية البشرية (Human Lymphocytes) خارج الجسم الحي

Authors: Moyassar Basil Hadi Al-Shaibani
Pages: 58-67
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Abstract

This study was designed to investigate the effect of different concentrations of Camellia sinensis methanol extract on the growth of human lymphocytes in vitro. The lymphocytes were isolated from healthy person free of any infection and unhealthy persons suffering from leukemia. The results showed that the extract of C. sinensis has no inhibitory effect on the growth of lymphocytes of the healthy person, or at least maintain their growth. At the same time, this extract has a distinct inhibitory effect on the total count of lymphocytes of leukemic origin when compared with the control that represents untreated human lymphocytes. It was concluded that the extracts of C. sinensis may play a role in maintenance the normal lymphocyte growth and possess inhibitory effect on the growth of human leukemic lymphocytes.


Article
Purification of Exotoxin A from Locally Isolate of
تنقية السم الخارجي من نوع A المنتج من قبل العزلة المحلية لبكتريا

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Abstract

Twenty-five isolates of Pseudomonas aeruginosa were isolated from wounds, burns, inflammation of the urinary tract(UTI), sputum and ear infections. These isolates were found producing exotoxin A as indicated by ELISA test. Isolates from different infections (burn, wounds and UTI) were selected according to their productivity. An amount of 0.045, 0.064 and 0.040 mg/ml protein which represent and 23.31, 29.73 and 19.01 ng/ml exotoxin A were produced respectively. Extoxoxin A was partially purified using precipitation by 90% ammonium sulphate followed by ion exchange chromatography with DEAE-cellulose then gel filtration chromatography by sepharose 4B was using to detection the molecular weight for the purified toxin was estimated as 65 KD.


Article
Alternative Chemical Treatments of Raw Water for Production the Drinking Water in Baghdad City
المعالجات الكيميائية البديلة للمياه الخام لانتاج مياه الشرب في مدينة بغداد

Authors: Ali Shihab Ahmed
Pages: 77-90
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Abstract

We found that the specification of raw water for Tigris river in Baghdad city ( approximately from the midpoint of water stream in the city ) was a good and need no effective treatments because the main pollutants are the turbidity and Total Suspended Solids (TSS) which represent the major pollutants only. These could be treated efficiently through the use of the new chemical treatment other than the alum and producing a large quantity of drinking water with high quality. The work included the collection and analysis of raw water samples from Tigris river during the first six months of 2011 at Alwahda project laboratories and our laboratories in university of technology . analysis results for raw water during this period showed that a number of determinants were within the acceptable range of the Iraqi drinking water specification ( especially the Total Hardness TH , 260 – 500 mg / l ; Alkalinity , 150 – 170 mg / l as CaCO3 and Total Dissolved Solids TDS , less than 800 mg / l) . The Turbidity and Total Suspended Solids ( TSS ) were not within acceptable values of Iraqi specification for drinking water and ranged between 14 -180 NTU and 27 – 362 mg / l respectively . We have used in our study a group of coagulant agents and flocculents like ferric chloride , wood ash and also the new manufactured organic compounds named ( XX-1 and poly XX-1 ) which have a good affinity to the heavy metals . The results showed that treatment the alum with ferric chloride at concentrations of 8 mg / l and 4 mg / l respectively and treatment with ferric chloride in concentration 8 mg /l were the best in decreasing the turbidity and TSS .The treatment with ferric chloride and the mixture of XX-1 and Ash gave negative results .The results showed the ability of synthetic organic XX-1 to remove the heavy metals from raw water , but have less ability to reduce the turbidity and TSS . It also observed the effect of ash in decreasing the turbidity and suspended solids when used with a alum .The clearness time was reduced to less than one hour after treatment with ferric chloride compared with alum (that use in Alwahda project ) . The results showed high efficiency for ferric chloride in reducing microbial contaminants especially the Total Viable Count (TVC) and coliform viable count . The effect of ferric chloride was started from 10 mg / l compared with the alum treatment . Microbial contamination decreased about 4 log by using 20 mg / l of ferric chloride to become 102 cell / 100 ml compared with the alum treatment (106 cell / 100 ml )


Article
Biotreatment of Al-Yarmook Hospital Wastewater Using Packed Bed Bioreactor
المعالجة الحيوية لمياه صرف مستشفى اليرموك باستخدام مفاعل حيوي نوع العمود المحشو

Authors: Mohammed B. Abdul Kareem
Pages: 91-100
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Abstract

Biotreatment systems of wastewater for hospitals are a challenge for environmental engineers. In this study, a pilot- scale system of aerobic fixed film packed bed was constructed for wastewater treatment of Al-Yarmok hospital which located in Baghdad. The aim of this study was to test the performance of fixed film packed-bed for elimination of the organic compounds from wastewater under aerobic condition. The parameters studied were: inoculums concentration, circulation rate, packing type and temperature. The results showed that the system efficiently removed about 85% of the chemical oxygen demand (COD) and 82% of Biological oxygen demand (BOD), while percent reduction in turbidity was about 93% and reduction in nitrate concentration was about 85%. It was found that temperature of 35oC and circulation rate of 16 L/min and inoculums concentration of 5% gave the best results for biotreatment of wastewater.


Article
Proposed Method to Determine the Possible Infection Dates with Visceral Leishmaniasis (Ka) in Central Iraq
طريقة مقترحة لتحديد تأريخ الإصابة المحتملة بمرض اللشمانيا الاحشائية(الكالاازار) في وسط العراق

Authors: Shihab Ahmed Salman
Pages: 101-105
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Abstract

This is a very simple and easy method to determine the possible infection dates with visceral leishmaniasis (KA) in the central region of Iraq. The scientific conception of this method based on the reported cases and the occurrence time of the vectors (sand flies) in the study area in the past three decades .This method may be helped to find more information in any program to control the disease based on the tracing of the incidence date in the endemic area.


Article
The Ability of Rapeseed (brassica napus l.) Seeds Oil in Inhibiting Cell Line Growth in Vitro.
قابلية زيت بذور نبات السلجم Brassica napus L. في تثبيط نمو الخطوط الخلوية خارج الجسم الحي.

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Abstract

The aim of the present study was assess the cytotoxic activity of seed oil on two cell lines (RD and L20B), through different exposure time (24 , 48 and 72 hrs.) and different concentration of seed oil (1.3 , 2.6 , 5.2 , 10.4 , 20.8 , 41.6 , 83.2 and 166.4 µg / ml) in vitro. The results showed that rapeseed ( Brassica napus L.) seeds oil has toxic effect on RD cancer cell line at high concentrations (83.2 and 166.4 µg/ ml) during 48 and 72 hrs. in comparison with the control. On the other side the result showed that there is no significant effect of these seed oil on the L20B transformed cells, except the treatment with higher concentration (166.4µg/ml) after 48 hrs. exposure time. In conclusion, the oil was shows selective (cell line – dependant) inhibitory effect on cancer cell lines and the plant may be a promising anticancer drug. Other biological properties should be appeared through the use of this oil extracted from the seeds of rapeseed.

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