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Iraqi Journal of Biotechnology

المجلة العراقية للتقانات الحياتية

ISSN: 18154794
Publisher: Baghdad University
Faculty: Institute of genetic Engineering and Techno-biology
Language: English

This journal is Open Access

About

Iraqi Journal of Biotechnology was founded in 2001 ,it was first issued in 2002,it is a semi-annual refereed scientific journal issued by the Institute of Genetic Engineering and Biotechnology in Baghdad University in fields of biology, environment, agricultural sciences ,medicine, dentistry, pharmacology, veterinary medicine and researches specialized in bioinformatics

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Contact info

E-mail:journal@ige.uobaghdad.edu.iq
www.iqjb.net
www.ige.uobaghdad.edu.iq : موقع المعهدعلى شبكة
الإنترنت
TEL:7789300
Baghdad-AL-Jadriyah -p.o.box:12074

Table of content: 2014 volume:13 issue:1

Article
In Silico Investigations of some Adverse Effects of Anticancer Drugs used in Iraq
دراسات حاسوب لبعض الجوانب السلبية للأدوية المضادة للسرطان المستعملة في العراق

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Abstract

Abstract: Adverse effects of anticancer drugs used in Iraq (30 drugs) were studied using in Silico approaches represented by using T.E.S.T v. 4.1 software. Results showed that 50% of drugs were mutagenic and 91% of them caused developmental toxicity .There was positive relationship between mutagenicity and developmental toxicity and the correlation coefficient was (r=0.779) . Most mutagenic activity caused by alkylating drugs (30.77%) and less than that by intercalating drugs (23.08%). Developmental toxicity mainly resulted from drugs affected the enzymes and receptors (29.41%) while drugs interact with tubulin resulted in (17.65%) of toxicity cases. The rest of percentages were due to other factors which some of them were unknown. There was no relationship between the number of rings (in general) in drug molecule and the mutagenicity and developmental toxicity. Other parameters such as number of rotatable bonds، HB donors and HB acceptors had no effect on mutagenicity and developmental toxicity.


Article
Detection of BRCA2 exon 10 genetic variations in Iraqi breast cancer patients
التحري عن التغايرات الجينية في المحور 10 لجين الـ BRCA2 في مرضى سرطان الثدي العراقيين

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Abstract

Breast cancer (BC) is the most prevalent malignancy in women in Western countries currently accounting for one third of all female cancers, and in Iraq rank the first among all cancers. Germline mutations in BRCA2 genes have been demonstrated to increase the risk of developing breast cancer. Conversely, the impact of BRCA mutations on prognosis and survival of breast cancer patients is still debated.Familial aggregation is thought to account for 5–10 % of all BC cases and germline mutations in BRCA1 and BRCA2 account for less of the half of these inherited cases. In Iraq breast cancer represents the principal death-causing malignancy among women, with (44.44 %) of the cases diagnosed before the age of 50 years. In order to study BRCA2 mutation spectra in the Iraqi population, direct sequencing of the entire coding region and intronicsequences flanking the exon was performed. A total of three BRCA2 sequence variants (a, 865A>C, N289H a, 1114 A>C. N372H a, 1153 A>G. K385E)were found. One of them a, 1153 A>G. K385E was novel. In conclusion, this study represents the evaluation of the deleterious and unclassified genetic variants in the BRCA2 gene exon 10 found in Iraqi population harboring of breast cancer.


Article
The role of matrix metalloproteinase -2 in bladder cancer
دور ماتريكس ميتالوبروتينيز-2 في سرطان المثانة

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Abstract

Transitional cell carcinoma of the bladder is the most common malignancy of the urinary tract.This study was designed to assess the expression of matrix metalloproteinase -2 in transitional cell carcinoma of the bladder using in situ hybridization technique.Formalin-fixed, paraffin embedded blocks tissue for thirty urinary bladder carcinoma patients were obtained from the archives of the Pathology laboratories of Al-Yarmouk and Baghdad Teaching Hospital from January 2011 to July 2012.In addition twenty apparently normal bladder autopsies were collected from the Forensic Medicine Institute archivesas control group.The blocks were subjected to cut as serial thin sections of (4μm) thickness and were sticked on positive charge slides to be used for In situ hybridization for the detection of MMP-2. Over expression of matrix metalloproteinase -2 was detected in 70.1923% (26 out of 30) of transitional cell carcinoma of the bladder samples (> 50% of the cells appearing as positive). The remaining 4 samples (42.775%) showed a weak- moderately expression (< 50% of the cells appearing as positive). In conclusion matrix metalloproteinase -2 play an important role in transitional cell carcinoma of the bladder.


Article
Quantitative Detection of Human Cytomegalovirus (HCMV) Gene in Iraqi Women with Pregnancy Associated Problems
التحري الجزيئي عن جين الفايروس المضخم للخلايا البشري في نساء عراقيات يعانين من مشاكل في الحمل

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Abstract

Cytomegalovirus (CMV) infection in pregnant women can be responsible for fetal loss or congenital malformation. In the present study CMV specific IgM and IgG antibody was detected by ELISA in pregnant women and its association with complication was assess. CMV specific IgG and IgM antibody was detected in 151(70.5%) of the 214 abortion case, these divided in two age groups they are ≤ 25 (56 cases(37%)), >25 95(63%) cases which are more vulnerable to infection) , these comprised of 142 (66.36%) recurrent and inevitable abortion, 2(0.93%)with intrauterine death, 7(3.27%) with abnormal child. Quantitative real-time PCR (RT- Q-PCR) assay for both age groups used for rapid and quantitative detection of HCMV DNA viral loud, where the concentration of virus was higher in women their aged more than 25. The study also showed patients with negative CMV antibody titer in absence of other causes. Suggesting the need for screening of women of child bearing age for CMV infection and RT-Q- PCR can be used to evaluate the clinical situation for the CMV infected women.


Article
Apoptosis and Necrosis Levels in Chemotherapy Treated Chronic Lymphocytic Leukemia Patients
مستوى الاستماتة و التنخر عند مرضى ابيضاض الدم المزمن المقاومين للعلاج الكيميائي

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Abstract

The results showed that CLL patients chemotherapy treatment increase the expression of Bcl2 gene (via measuring the phosphatidylserine -PS level) which increased apoptosis and necrosis percentage. It is also showed that the Necrotic cells (%) were found to be significantly higher in patients samples (1.07 ± 0.03) than in healthy (0.17 ± 0.02) with no significance in viable cells (%) (82.88% in patients and 86.8% in healthy). The apoptotic cells % were found to be significantly higher in patients (15.04 ± 1.03) than healthy (4.40 ± 0.5). On the other hand, late apoptosis % showed no significant results (0.639 % in patients and 0.803 % in healthy).

Keywords

CLL --- Apoptosis --- Bcl2 --- Mutations --- PCR


Article
The Effect of Heptachlor on Histology of Seminiferous Tubule and Mice Testis Activity
تاثير الهبتاكلور على النبيبات المنوية نسجيا وعلى نشاط خصى الفئران

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Abstract

The study was aimed to illustrate the effect of heptachlor on seminiferous tubule and spermatic cells in the testis of mice .Heptachlor 10 mg / kg was given daily by gulping for five weeks. Mice were sacrificed after injected with colchicines in traperitonially , testis were collected after 7,21,36 days to study the effect of heptachlor on histo-testis and improved heptachlor activity not as chemical mutagen but as a tumor promoter by act directly on DNA which reflected on decreasing testicular Leydig cell and spermatogenic deficiencies when comparison with control and vitamin C.


Article
Contribution of IL-10 (SNP -819 C/T and SNP-1082 G/A) polymorphisms variants to the risk of type 1 diabetes in Egyptian population
مساهمه التباين الجيني لجين الانترلوكين- 10 و(SNP -819 C/T SNP-1082 G/A) على مخاطر الاصابه بمرض السكري من النوع الاول في المجتمع المصري

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Abstract

This study investigated whether interleukin- 10 (IL-10) gene promoter region polymorphisms are associated with susceptibility or clinical presentation of type 1 diabetes. The frequency of -1082G/A and -819C/T, polymorphisms was analyzed in 60 Egyptian patients with type 1 diabetes and in 60 healthy control subjects in a case-controlled study. However, the -819C/T not showed significance between patient and control (P=0.208) and 1082G/A were associated with T1DM disease (P<0.0001). These results suggest that the IL-10 gene promoter polymorphisms are associated with type 1 diabetes in Egyptian population.


Article
PCR-based approach to detect the Prevalence of exo S. Gene in Local Isolates of P. aeruginosa isolated from Otitis
استخدام تقنية التفاعل البلمري للتحري عن الجين .exo S المشفر للذيفان الخارجي في عزلات محليه من بكتريا الزائفة الزنجارية المسببة لالتهاب الاذن

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Abstract

This study was designed to detect exo S. gene in local isolate of Pseudomonas aeruginsoa using PCR technique. It includes 49 isolates of bacteria diagnosed by classical methods, DNA isolated and subjected to amplify using specific primers for bacteria. The results of PCR amplification for the gene encoding for exotoxin S indicated that out of the forty nine local isolates of P. aeruginosa enrolled in this study, thirty nine (39) isolates showed the presence of the genes encoding for exotoxin S corresponding to 79.5% of the isolates (629 bp PCR products) while only ten (10) isolates corresponding to 20.5% of the total forty nine (49) isolates were recorded to be negative. With a result of 72% of the bacterial isolates demonstrating positive reaction for the presence of exo S., it can be concluded that exo S. is a prominent virulence factor for P. aeruginosa that is expressed along the infection process.


Article
Study of Chromosomal Aberrations and Micronucleus Formation in Some Iraqi Patients infected with Acute Myeloid Leukemia (AML)
دراسة التشوهات الكروموسومية وتكوين النويات الدقيقة عند بعض المرضى العراقيين المصابين بإبيضاض الدم النخاعي الحاد

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Abstract

The effect of acute myeloid leukemia (AML) was studied on some cytogenetic parameters occur in lymphocyte in three cases; before, during and after chemotherapy treatment. The study involved 47 AML Iraqi patients their ages ranged between 2.5-81 years including 20 females and 27 males. Results were compared with 26 healthy individuals and summarized as follows: 1- AML was the most common leukemia in adults as compared with children. It was found that 46.8% AML patient ages were less than 15 years; 90.15% at 30-16 years; 40.9% at individuals at 31-45 years, the percentage increased to 90.5% in those who are over 45%. 2- AML was more common in males than females recording 57.5% and 42.5% respectively; representing 1.35:1.00 male:female ratio. 3- Two cases representing 5.3 of the studied sample exhibited dioploidy after examination under light microscope. The highest mitotic index was 7.498% and occurred in patients before taking therapy compared to 6.784% during therepy and 7.000% after therapy. 4- Micronucleus mean values recorded 0.033, 0.020 and 0.036 MN/1000 cells for AML patients before, during and after therapy respectively, when compared with the control which recorded 0.002 MN/1000cells. Nuclear division index (NDI) means before, during and after therapy of AML patients were 1.658, 1.000 and 1.424 respectively compared with control which recorded 1.282.

Keywords

AML --- CA --- MI --- MN --- NDI

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