The effect of sonicated Cryptococcus neoformans & Salmonella typhimurium Killed whole cell antigens on cellular immune response in rats


Abstract: The research was included to prepare the Sonicated Cryptococcus neoformans and killed whole cell of Salmonella typhimurium antigens and evaluate their effects on the cellular immune response by using DTH-skin test and Lymphocyte /Neutrophil ratio in rats. The rats were divided randomly into four groups (1st group: contain 20 rats were immunized S/C with KWCA-S(9x108 cfu/ml) + KWCSA-C (1000μg/ml), 2nd group contain 20 rats were immunized S/C with KWCA-S (9x108 cfu/ml)+ KWCSA-C (500μg/ml), 3rd group immunized S/C with KWCA-S ( 9x108 cfu/ml) only, 4th group was inoculated S/C with PBS (pH 7.2) and considered as control group. There was significant differences (P<0.05) between 1st; 2nd and 3rd groups compared with control group in rat's footpad thickness. Also there was a significant differences (P<0.05) at day 50 for immunized groups compared with day 10 in Lymphocyte/Neutrophil ratio.