Antibacterial Pattern of Lactic Acid Bacteria on Streptococcus Mutans Isolates from Dental Caries

Abstract

Several approaches have been taken in the prevention of dental caries; the main approach is to render enamel surface more resistance to attack of acidogenic bacteria. In this study one hundred dental plague samples with 20-30 mg of plagues were collected. These patient's samples were collected during March to May 2014 from Baghdad dental clinics. Culturing of dental plagues was inoculated on different selective, enrichment and diagnostic media to be obtain pure culture of Strep. mutans. Streptococcus mutans is considered the main cause of destruction of dental tissues, with the incidence 13%. The inhibitory effect of Lactobacillus gasseri against Streptococcus mutans isolates was studied by well-diffusion method on solid media. The results showed that the Lactobacillus gasseri has a highly effects of inhibition zone on the Strep. mutans growth (92.30%) and the inhibition diameters zone were more than 12 mm. Also this effect compared with other antibacterial drugs, which were showed that the isolates had different responses of resistance to Ampicillin (AM) 5(38.4%), Azithromcin (AZM) and Lincomycin (LN) 2(15.4%) for each, Gentamycin (CN) 4(30.7%), so that the Cefotaxim (CTX) antibacterial recorded lowest resistance 1(7.6). Hence, this study reveals the potential of Lactobacillus gasseri as an alternative and complementary treatment medicine for dental caries by inhibiting the growth of Streptococcus mutans. Also these results may provide a basis for alternative therapies to prevent or treatment of dental caries.