Embryo Grade I is The Most Important ICSI Parameters That Predict Intracytoplasmic Sperm Injection(ICSI) Outcome

Abstract

The aim of this study was to compare different intracytoplasmic sperm injection (ICSI) parameters with its outcome and predict the most important factor that relate with successful rate.A total seventy-five infertile women aged between 22-45 years(31.43 ± 5.38 years), referred to the fertility clinic in Al-Sadder teaching hospital, , and undergone intracytoplasmic sperm injection throughout period from March 2013 to January 2014,were included in this study. Fertilization rate (FR) and cleavage rate was calculated. The embryos grading was evaluated according to their morphology and percentage of fragmentation. The biochemical pregnancies was confirm on the fourteenth day of embryo transfer by measurement of serum human chorionic gonadotrophine ( B- HCG ). Out of the 75 included patients, 15 (20%) achieved pregnancy after ICSI. Pregnant women were associated (P < 0.05) . Total oocyte retrieved , stage metaphase II oocyte , pronucleus , cleavage rate , grade I ,total number of embryos ,and number of transferred embryo significantly higher in pregnant women when compared with non-pregnant women(P<0.05) Analysis of ROC curve revealed that area under the curve for number of grade I embryos was 0.868 for predicting pregnancy followed by followed by MII (0.815), number of total oocyte .retrieved and PN (0.773), ET( 0.767) and total number of embryos(0.758) respectively.Data show that the number of grade I embryos is the better at predicting for successful pregnancy outcome.(higher test result give more positive test).