Incidence and Antibiotics Sensitivity of Multidrug-Resistance of Pseudomonas aeruginosa Isolated from Burn’s Patients and Environmental Samples fromThree Hospitals in Baghdad

Abstract

Two hundred swab samples were collected from burn patients and burn units for the period, beginning from August 2012 to the end of April 2013 from a number of hospitals in Baghdad governorate including: Al-Kindi General Teaching Hospital, Al-Yarmuk General Teaching Hospital and Al-Imam Ali Hospital. The collected samples were cultured on different media and tested biochemically in order to find out the profile of bacteria that colonize burn patients wounds and environment of burn units. The results of bacterial culturing revealed that out of 200 samples, 105 samples 52.5% were observed to have bacterial growth (positive samples), while negative samples represented 95(47.5%). The profile of the bacteria in the cultured samples revealed: Pseudomonas aeruginosa 40.95% was the most common isolate followed by Staphylococcus aureus 20.0%, Klebsiella pneumoniae 17.1%, Escherichia coli 8.5%, Pseudomonas putida 4.76%, Enterobacter aerogenes 3.80%, Acinetobacter baumannii 2.85% and Proteus mirabilis 1.90%. Forty three P. aeruginosa isolates were tested for antibiotic susceptibility. The results showed most isolates were potentially resistant to different antibiotics as follow, all isolates 100% had resistance to Ceftriaxone, Cefepime, and Chloramphenicol, and showed high resistance to Tobramycin 95.3%, Gentamicin 93.0%, Ceftazidime 88.3%, Cefotaxime 86.0%, Piperacillin 83.7% and Amikacin 79.0%, beside illustrating low resistance to Aztreonam 67.4%, Ciprofloxacin 46.5%, and Imipenem 13.9% among these antibiotics, Imipenem was the most effective antibiotic because 86.0% of the isolates appeared to be high sensitive to it.