Immunohistochemical expression of MMP1 and TIMP1 as markers of migration in Hodgkin’s and non-Hodgkin’s lymphoma of the head and neck region (A comparative study)


Background: Malignant lymphoma is a term that describes primary tumors of the lymphoreticular system, almost all ofwhich arise from lymphocytes.MMP-1 is the most ubiquitously expressed interstitial collagenase, a subfamily of MMPsthat cleaves stromal collagens. It is also called collagenase-1.TIMPs which inhibits MMP activity and thereby restrictextracellular matrix breakdown, TIMP-1 is a stromal factor that has a wide spectrum of functions in different tissues.Material and Methods: This study was performed on (68) formalin-fixed, paraffin-embedded blocks,histopathologically diagnosed as lymphoma (head and neck lesions). Immunohistochemical staining of MMP1andTIMP1 was performed on each case of the study sample.Results: The expression of MMP1was cytoplasmic, the study cases showed a 98.5% positive reaction to MMP1, score 3was the most common and found in 60.3% of all cases.The expression of TIMP1was cytoplasmic, 92.6% of casesexpressed positive reactions to TIMP1, score 1 was the most common and found in 57.4% of all cases.Conclusion: This study showed for the first time the effect of MMP-1 in HL, which is considered to be as an invasiveand migratory cell marker. A significant difference was found among the subtypes of NHL in relation to TIMP1,TIMP1inhibits the effect of MMP1 and as MMP1 is elevated the TIMP1 will be elevated too