عزل وتشخيص أنواع جنس .Aspergillusspp من الجهاز التنفسي للمصابين بالأمراض الرئوية المزمنة

Abstract

Study aims to isolate isolates fungus Aspergillus spp. Isolated from injuries chronic lung in the province of Diwaniyah, where the samples were collected sputum from patients clinic Advisory diseases thoracic and respiratory, and subjected the samples to a microscopic examination of direct and diagnosis pea and molecular technology, PCR, and the results showed (107) isolation of its ownership to the genus Aspergillus spp. and by (28.08%) of the total isolates and adult (381) isolation, while the level of species belonging to the genus Aspergillus spp. record A. fumigatus highest percentage of frequency (29.9%), followed by the fungus A. niger (28.9%), as well as the isolated species A. flavusand A. terreus and A. nidulans(18.7%, 12.14%, 2.8%), respectively, have also been isolated species A. ochraceus, A.parasiticus, A.penicillioides and A.ustus which are considered according to available studies has been isolated for the first time in the city of Diwaniya. The results also showed the level of diagnosis of type that molecular diagnostics has proved highly efficient in the diagnosis.