EVALUATION OF THE ISOLATION AND DETECTION METHODS FOR SALMONELLA SPP. FROM EGG SHELL CONTAMINATION USING MULTIPLEX PCR.

Abstract

A total of seventy four egg samples were collected from different markets sources, toinvestigated for Salmonellaspp. the bacteria was isolated and identified using culturing onselective media, in addition to, biochemical and serotyping by monovalent antisera.Multiplex Polymerase Chain Reaction(PCR) detection of theinvA; and DH genes wasused for conformation of the identification of the Salmonella spp.. Twenty eight samplesfrom seventy four eggs provide positive results with PCR as Salmonella spp. dependingon InvA gene which is the target for the identification at the genus level.