Immunological methods to develop camelid anti-cancer

Abstract

Background: The humoral immune response of the Camelidae is unique as these animals are the only known mammals that seem to possess functional homodimeric heavy-chain antibodies besides the classical heteromeric antibodies composed of heavy (H) and light (L) chains. Objective: to assess the ability of camel IgGs to react much better to the tumor associated antigen (TAA) than human IgGs and independently from the haplotype-restriction of the TAA complexes with MHC molecules. Materials and methods: The immunization of carcinoembryonic antigen (CEA) of two adult camels was carried out in the private farm at Sharjah – UAE for five weeks and then the specimens were collected and transferred to the central laboratory of the Sharjah University. The project was extended from April 2008 to April 2009. The specimens were fractionated by chromatography then were evaluated by using Elisa technique.Results: The results of CEA immunization of adult camel and its calf was determined by using indirect ELISA test. The ELISA readings showed positive response with camelid serum, urine, milk and calf serum. Negative results showed with pre-immunized serum from camel and its calf and from blocking buffer.Conclusions: This work succeeded in formulating TAA-specific highly reactive camelid IgG that are able to recognize only the cancerous cells and sparing the normal cells. These TAA-specific antibodies bound tightly to TAA antigens as well as to control carcino-embryonic antigen (CEA).