Impact of Laser Induced Forward Transfering on Transfer of Escherichia Coli Bacteria


Laser Induced Forward Transfer (LIFT) is a technique uses to print different materials with high spatial resolution for microarray preparation. In this work, the mechanism in which LIFT process effect E.coli bacteria used to produce microarray droplets from a liquid solution thin film coated a glass slide previously coated with gold of 65nm thick which was used as a laser absorbing layer. The transferred bacteria was achieved at laser fluence of 10 J/cm2 and 100 µm distance between donor and receptor substrates.The purpose of using such a technique was to assess the impact of laser on the viability of the transferred bacteria. Scanning electron microscope observation indicated that E.coli bacterial cells integrity was maintained during the process. The bacteria was viable as tested using MacConkey agar culture media.