The Relevance of Adiponectin and Resistin Levels with Oxidative Stress in Insulin Resistant Type 2 Diabetes

Abstract

Objectives: The present study was designed to verify the changes of adiponectin and resistin with oxidative stress in insulin resistant in diabetes mellitus type 2.Methodology: To achieve this aim 60 patients with type 2 diabetes mellitus (24 male and 36 female ) of ages, 53.9 ± 12.4yeares in addition to 30 apparently healthy individuals (13 males and 17 females) were enrolled . The concentration of fasting blood glucose , triglyceride , insulin , adiponectin, resistin and malondialdehyde (MDA) levels were measured. Fasting insulin concentration, adiponectin and resistin was measured by enzyme linked immunosorbantassay( ELISA) . Fasting blood glucose and triglyceride levels were determined by enzymatic methods. Malondialdehyde (MDA) levels were measured by spectrophotometericmethods . Insulin resistance was evaluated by four methods .Results : The results indicated that out of 60 patients , 49 (81%) , 45 (75%) , 33(55%) and 32 (53%) were insulin resistant when they were evaluated by Homeostasis model assessment( HOMA) , Quantitative insulin sensitivity check index (QUICKI) , McAuley's index (MCA) and fasting insulin(FI) indices respectively . The 49 insulin resistant type 2 diabetic patients (IRP) that obtained through the HOMA method were assessed for the alteration in the levels adiponectin ,resistin and MDA . The analysis of the data revealed a significant decrease of adiponectin in the IRP group when compared with those of the insulin sensitive patients (ISP) and the control groups . Significant elevation of resistin levels were observed in the IRP group with respect to those of the control groups .Conclusion: Insulin resistance was found to be significantly positively correlated with resistin, MDA and significantly negatively correlated with adiponectin levels in diabetic patients . Adiponectin exhibited insignificant negative correlation with resistin and significant negative correlation with MDA levels, while the level of resistin showed a significant positive correlation with MDA level in insulin resistant type 2 diabetics .Recommendations: A study on of the effect free fatty acid on the activity of insulin degrading enzymes in obese individual .