Clinical Investigation of The Role of Tumor Necrosis Factor-α and Other Risk Factors In The Evolution of Breast Cancer in Kerbala City

Abstract

Background :Breast cancer is the most common malignancy in females worldwide .It is a leading cause of death in women. The tumor markers CEA ,CA 19-9 and CA15-3 and inflammatory cytokine TNF-α were shown to contribute to breast cancer development and metastasis.Objective: In this study, we wished to determine whether there are associations between these factors along stages of breast cancer progression, and to identify the possible implications of these factors to disease course. Materials and Methods: Forty female patients with Breast cancer with ages ranged between (29- 68) years were taken from (Al-Hussein Medical City/Kerbala).Control group consisted of 20 healthy people who were free from signs and symptoms of breast cancer who matched in age and gender with patients, and had no history for any cancer problem. TNF-α(TNF-α EASIA Kit, DIA source) was studied using the enzyme-linked immunosorbent assay (ELISA) method, Estimation of CEA,CA19-9 , CA15-3 by Enzyme-linked fluorescence assay (VIDAS. Biomerieux SA/France). T-test, ANOVA and Pearson correlation used to analyze results by using SPSS version 20. P-value ≤ 0.05 was considered significant. Results: TNF-α ,CEA ,CA15-3 levels increased significantly (p< 0.05) in patients compared with control group. But the level of CA19-9 does not show significant increase when compared with control groups. Conclusion :Significantly correlation of TNF-α, CEA and CA15-3 with breast cancer but there is no significant correlation withCA19-9. So no significant correlation after chemotherapy