Serological diagnosis of thyroid disorders in patients using minividas TsH, T3, T4 Enzyme linked fluorescent immunoassay (ELFA)

Abstract

Serum samples 363, 243 and 234 for measuring the level of TsH, T3 and T4 respectively were taken from patients of both sexes clinically suspected with the thyroid disorders using enzyme linked fluorescent immunoassay (ELFA) for measuring TSH, T3 and T4. Results were showed that out of 363 serum samples only 36 (9.88%) were positive for the hyperthyroidism, the level of TSH were < 0.15 miu/ml accompanied by raised level of T3 > 2.33 nmol/L in 38 (15.6%) and raised level of T4 > 120 nmol/L in 32 (13.6%). And out of 363 serum samples only 31 (8.5%) were positive for primary hypothyroidism, the level of TsH were > 7 miu/ml accompanied by low levels of T3 <0.92 nmol/L in 18 (7.4%) and low level of T4< 60 nmol/L in 27 (11.5%). Three (0.82%) serum samples were positive for secondary hypothyroidism accompanied by low level of TsH together with subnormal level of T3 and T4. Euthyroidism cases were positive in 293 out of 363 (80.8% serum samples examined with normal level of TsH 0.15-7 miu/ml and normal level of T3 and T4 (0.92-2.33 and 60-120 nmol/L) respectively. Conclusion:1.Minividas TsH, T3 and T4 enzyme linked fluorescent immunoassay (ELFA) are of value to confirm clinical diagnosis of thyroid disorders.2.Hyperthyroidism disorder were present in 9.88%, hypothyroidism (primary and secondary) were present in 8.5%, 0.82% respectively, where as Euthyroidism were present in 80.8% of serum samples examined.

Keywords

thyroid, TsH, T3, T4