Association of The T45G Polymorphism of Adiponectin Gene with Polycystic Ovary Syndrome in Women of Babylon Province/ Iraq


Polycystic Ovarian syndrome (PCOS) is being the most frequent cause of anovulatory infertility, Adiponectin is the most abundantadipocytokine and may play a role in the regulation of insulin sensitivity and IR in PCOS and count for 0.01% or 3–30 μg/ml of total plasma proteinthe adiponectin gene contains 3 exons spans 16 kb on chromosome3q27.The aim of the present study was to evaluate the genetic influence of the adiponectin gene polymorphisms in the development of PCOS among women of Babylon Province/ Iraq. sixty three women were studied ,and were classified into two groups of : first group consists of 32 women infected with polycystic ovaries syndrome, the second consists of 31 healthy women to detect the presence of T45G polymorphism within the gene. From all subjects a whole-blood sample was taken and was used for isolation of peripheral blood leukocytes. The adiponectin T45G polymorphism, located in exon 2, was genotyped by amplification of genomic DNA.The present study included study of the relationship of this gene with PCOS women in reproductive age, A statistically significant difference was observed in the frequency of TT,TG and GG genotypes between women with PCOS and controls .