Spectrophotometric Determination of Amoxicillin Trihydrate in Pure and Pharmaceutical Dosage Forms

Abstract

A rapid and sensitive method for the determination of amoxicillin trihydrate (AMXT)based on the diazo-coupling reaction was studied. Sulphanilic acid diazotizes with nitrite ionin acidic medium to produce a water soluble, colorless diazonium ion, which subsequentlycoupled with AMXT to form a colored azo dye in the alkaline medium, having maximumabsorption at 455 nm. The calibration graph showed that Beer's law is obeyed over theconcentration range of 0.3 – 30.0 μg/mL of AMXT, with the detection limit of 0.15 μg/mLand molar absorptivity was 2.3 × 104 L/mol.cm. The accuracy and the precision wereacceptable depending upon the values of error percentage and relative standard deviation.The influence of common interferences was studied and the method was applied with goodrecovery for the determination of AMXT in pure form and different pharmaceuticalformulations, which commercially available in Erbil market