انتشار وانتاج السم المعوي enterotoxin B والحساسية للمضادات الحيوية لعزلات جرثومة المكورات العنقودية المعزولة من الاسهال

Abstract

One hundred and seventy five samples were collected from diarrhea swabs, twelve isolates (6.7%) were obtained and diagnosed as Staphylococcus aureus. The production of enterotoxin B were tested by using reverse passive latex agglutination. Eight isolates (66.7%) had the ability to producing enterotoxin B. S. aureus were 100% resistancy were recorded for two antibiotics, ampicillin and penicillin, while in the opposite direction, 100% sensitive to Fusidic acid . Graded resistant was observed in other antibiotics, include: 66.7% for erythromycin, tetracycline, oxacillin and methicillin. The results of this study showed the wide spread of enterotoxigenic and multidrug-resistant S. aureus isolates isolated from diarrhea samples. The lowest temperature at which SEB production manifested was 20°C in four isolates (SA2,SA4,SA11 and SA12), while the highest temperature at which SEB was produced was 45°C, in three isolates (SA2,SA11 and SA12). The lowest pH value at which SEB production was still observed was 4 in two isolates ( SA4 and SA12) . These isolates were able to produce SEB even at pH 10.5, which was the highest pH value among the isolates studied. SEB is produced within a wide pH range but the influence of temperature is an essential one for the production of this toxin.