Direct detection of Shiga toxin producing by Escherichia coli by real-time PCR

Abstract

Shiga toxin-producing Escherichia coli (STEC) are defined as strains of E. coli that produce Shiga toxins (stx), which known as important causes of diarrhea in sheep and cattle. This study was conducted to determine of Shiga toxin 1 producing E. coli isolates from diarrheal samples of sheep and cattle. Samples were collected from different fields in Diwanyia city. A total of 50 diarrhea samples (25 of sheep and 25 of cattle) were subjected to bacterial DNA extraction by using (AccuPrep® Stool DNA Extraction Kit). The extracted DNA subjected to Real-Time PCR technique for detection of Shiga toxin 1 (stx1) gene. Results display that sheep are more prevalence to shedding the Shiga toxin 1 producing E. coli in (5/25) (20%), while in cattle (2/25) (8%) positive samples. In conclusion the using of Real-Time technique was shown high specific and rapid method in direct detection of (stx1) gene and most the sheep and cattle which infected by diarrhea carried the Shiga toxin 1 producing E. coli.