The role of Betaine and Choline as a substitute for Methionine in diet and its effect in biochemistry of broiler chickens blood

Abstract

This study was conducted at the poultry farm, department of Animal Resources, Collegeof Agriculture /University of Al-Qasim Green. The field work of study was performed duringthe period from 20/2/2014 until 2/4/2014 and then followed by the laboratory work. Theproject of this study was aimed to identify the effect of utilization of betaine and choline andtheir synergistic mixture to meet the deficiency of methionine which could happen on thediets effect in biochemistry of broiler chickens blood .Three hundred unsexed broiler chickens strain of the commercial Rose (Rose-308)was used in this experiment. The chicks were reared on a letters floor and treated from the ageof one day until 42 days. The chicks were randomly housed into 5 treatments and by 4replicates per treatment, and each replicate was included 15 chicks , the treatment as thefollowing:1. (T1): positive control, which contain methionine as recommended by AviganCompany.2. (T2): negative control, which contain half the requirements of methionine asrecommended by Avigan Company.3. (T3): T2 and betaine (800mg/kg feed).4. (T4): T2 and choline (800mg/kg feed).5. (T5): T2, betaine (400mg/kg feed), and choline (400mg/kg feed).The most important results of this study could be summarized by the following:1. Significant elevation (P< 0.05) in glucose concentration was observed for T2treatment birds compared with T1 and T3 treatments. Whereas no significantdifferences were observed among T1, T3, T4, and T5 treatments.2. Significant reduction in the blood cholesterol concentration for T3 and T4 treatmentswere observed compared with T2 treatment. Whereas no significant differences wereobserved among T1, T3, T4, and T5 treatments and among T1, T2, and T5 treatmentsas well.3. Significant elevation (P< 0.05) in the total protein concentration and a significantreduction (P< 0.05) in uric acid concentration was taken place in both T1 and T3treatments birds compared with T2 treatment. Whereas there were no significantdifferences noticed among T2, T4, and T5 treatments.