Assessment the Level of Interleukine-5 and Eosinophils Count in Allergic Rhinitis and Asthma Patients

Abstract

Fifty blood samples have been collected from patients attending the center of the allergy and asthma in the province of Babylon ( 25 before immunotherapy treatment and 25 after treatment ), in addition to 10 samples from non-allergic persons as a control . IL-5 concentrations have been measured by using ELISA, while the eosinophils have been measured by using complete blood count device. This results shows that there is an increase in the level of IL-5 and eosinophils count before allergen-specific immunotherapy treatment for allergic rhinitis patients which reached 53.1402 ± 3.67801pg/ml and 0.8249 ± 0.20108 cell/mm3 respectively and asthmatic patients 49.8922 ± 7.43190 pg/ml and 0.6523± 0.25712 cell/mm3 respectively in compared with their level after treatment for allergic rhinitis patients which reached 22.7315 ± 4.03680 pg/ml and 0.3642 ± 0.14734 cell/mm3 and asthma patients 22.7177 ± 4.13433 pg/ml and 0.3645± 0.15743 cell/mm3 respectively, and for control which reached 24.5404 ± 8.64920 pg/ml and 0.2252 ± 0.09139 cell/mm3 respectively. The results showed that there were an increase in the level of IL-5 for allergic rhinitis patients before and after treatment in compared with asthma patients , eosinophils increase in allergic rhinitis patients in compared with asthma patients before immunotherapy treatment, but the opposite is true for their count which increase in asthma patients in compared with allergic rhinitis patients after treatment. The level of IL-5 and eosinophils count decrease gradually by increasing the period of immunotherapy treatment which divided into three groups two months , six months and one year , the level of IL-5 for these groups 26.2821  3.81978 pg/ml , 21.3737  2.47124 pg/ml and 20.5029  4.67899 pg/ml respectively , while the count of eosinophils for these groups reached 0.4567  0.15057 cell/mm3 , 0.4282  0.15162 cell/mm3 and 0.26270.06517 cell/mm3 respectively.