In vitro assessment of L.acidophillius and L.casei binding capacity against aflatoxins B1

Abstract

The aim of this study was to investigate the efficacy of Lactobacillus acidophilus and Lactobacillus casei for the binding of aflatoxin B1 in vitro in aqueous solution. We have chosen to focus on those bacteria due to their long history of use as a probiotic, the availability of a bank of strains with documented genetic diversity isolated from various ecological niches, and the availability of genetic tools to examine mechanisms of probiotic activity. The Lactobacillus acidophilus exhibited different degrees of aflatoxin B1 binding, the highest AFB1 binding was L. acidophilus 20079, which bound 89.4% in probiotic condition and 87.14% in prebiotic condition of the available AFB1 (10 ppb) after 4h of incubation time at pH6.0. The L. casei 20011 bound 82.87% in probiotic condition and 81.24% in prebiotic condition after 4 h of incubation time at pH6.0. The incubation time (2 and 4 h) affect the elimination of the toxin as significant differences in the amount of AFB1 removed by L. casei and L. acidophilus were observed.