Role of Osteoprtegerin levels in a sample of Iraqi diabetic foot patients

Abstract

Diabetic foot (DF) is serious diabetes (DM) complication leading to delayed wound healing and may result in amputation. Osteoprotegerin (OPG) is a glycoprotein decoy receptor of RANKL(OPGL), involvement of OPG in the pathogenesis of diabetic complications has been proposed. Interactions OPG/OPGL has been proposed to be a majorinhibiter of osteoclastogenesis (differentiation of osteoclasts cells),and OPG also has a role in the regulation of the immune response . This study was done to investigate whether the serum levels of circulating soluble osteoprotegerin, glycoprotein associated with the apoptosis, are altered in patients with type 2diabetes whom have diabetic foot ulcerations. Osteoprotegerin was measured with the ELISA method in thirty (30) normal controls (groupI), twenty five patients with type 2 DM (duration ranged from 1-32 years (group II) and in twenty five diabetic foot patients with type 2 DM (duration ranged from 2-31 years (group III). Also, serum glucose (Fasting), lipid profile (total cholesterol, riacylglycerol, HDL-c,LDL-c and VLDL-c) and hs-CRP were determined after 12hrs of fasting. Results: The patients with diabetic foot lesions were found to be poorly controlled and had significantly higher levels of fasting Plasma glucose (FPG) (p<0.05,p<0.001) when compared to patients whom without diabetic foot lesions and control groups. Also the patients with diabetic foot lesions were found have significantly higher levels of OPG when compared to patients without diabetic foot lesions and healthy control groups (p<0.01,P<0.001)respectively . Serum levels of total cholesterol, triacylglycerol, LDL-c and VLDL were significantly higher (p<0.05) in diabetic patients (with and without foot ulceration) in comparison with healthy normal control, while HDL-c level was lower in both groups than in the control group but not reach statistical significant. There are no significant different in lipid profile between diabetic foot patients and without diabetic foot patients. OPG serum levelswere significantly increased in diabetic foot group as compared with patients whom without diabetic foot and normal control (10.82±2.87 ,6.16±6.18, ng mL