Optimum conditions for asparaginase production by Serratia marcescens S10-1

Abstract

Optimum conditions for asparaginase production by Serratia marcescens S10-1 was studied. Results showed that there was an overproduction obtained includes supplimentation of the production medium with 3% maltose (as a sole source for carbon and energy) , and 2% pepton (as a nitrogen source) , and a mixture of phosphate source 0f: K2HPO4 and KH2PO4 in a ratio of (1 : 1) in a concentration of 0.2 % , pH of the production medium was adjusted to pH 7.5 , inoculated with inoculum size of 2.2 × 108 CFU / ml and incubated at 37 °c for 24 hours in shaker incubater at 150 rpm . Under these conditions enzyme specific activity of asparaginase produced by S. marcescens S10-1 mutant was reached 57.34 U/ mg.