Detection of Y Chromosome Microdeletion Region in Infertile Men Need to Conceived with ICSI


With the advancements in the field of assisted reproductive technologies including intracytoplasmic sperm injection (ICSI), it is necessary to detect the presence of y chromosome microdeletion regions (AZF) Azoospermia factor which is considered the main genetic causes of male infertility. The current study was conducted to achieve the genetic factors in infertile men needed to conceive with ICSI by detection of Y chromosome microdeletion regions usingpolymerase chain reaction (PCR). Results revealed that out of 24 cases of infertile men 4 cases (16.67%) were positive to the presence of y chromosome microdeletion regions represented with AZFc region(100%) including SY255 and SY254 genes while, 20 cases (83.33%) were negative to presence of microdeletion regions in infertile men. AZFb and AZFa were not detected.