The protective effect of vitamins A, D3 and E on testicular damage induced by ketoconazole in male mice


The study was aimed to evaluate the protective effect of the combination of multivitamins AD3E against the testicular injury induced by using of ketoconazole in male mice. Eighteen adults male albino mice (Musmuscullus) weighing about 25 gm and aged 60 days were utilized in this study. Animals were kept in same environmental conditions, and divided into three equal groups. Control group has been given olive oil (0.2 ml) orally by gastric gavage tube, and at the same time given normal saline (0.125 ml) injected intra peritoneally once daily for 10 successive days and consider as placebo. T1 group treated with ketoconazole 100mg/kg BW administered orally plus intra-peritoneal injection of (Vitol-140®) a combination of vitamins AD3E in a dose of 400,000 IU/kg BW vitamin A, 200,000 IU/kg BW vitamin D3, and 100mg/kg BW of vitamin E once daily for 10 successive days. T2 group were treated with ketoconazole alone in a dose of 100mg/kg BW given orally for 10 successive days. After 10 days animals were sacrificed, orchiectomy was performed, and the testis was processed for histopathological examinations with light microscope after staining with (H&E) stain. Calculation of spermatogonia, primary and secondary cells were counted for 20 seminiferous tubules in treated and control groups. In T2 group there was massive destruction of the testicular tissue characterized by abnormal splintered architecture of the tissue. The cellular mass and density of the tubules were suppressed accompanied by a suppression of spermatogenesis process within the seminiferous tubules, with vacuolation of spermatogonia and presence of multinucleated spermatid giant cells. Also there was few number of Leydig cells between the seminiferous tubules in compared with control group. While in T1 group there was complete spermatogenesis within the seminiferous tubules. There were normal spermatogonia, primary and secondary spermatocytes and spermatid. Also there were a high numbers of Leydig cells between the seminiferous tubules, and the fields resemble that of control group. The number of primary and secondary spermatogonia was registered a significant difference between control and the treatment groups in all studied parameter (spermatogonia, the primary and the secondary cells). The mean number of spermatogonia in control group was significantly higher than (T2) group while no significant differences between control and the (T1) group. The mean number of primary and secondary spermatocyte show significant difference between control and (T2) groups. also there was a significant difference between (T1) and (T2) groups.