Extraction and purification of outer membrane (porins) from Pseudomonas species bacteria and different cases of patient

Abstract

There are important role in the effect of porins to the bacterial sensitivity and resistance to antibiotic. Three isolates were selected from bacteria P. aeruginosa, and two isolates of bacteria P. fluorescens and P. oryzihabitans, respectively, as a source of extraction, purification and characterization of the outer membrane proteins (porins). These isolates were selected based on their sensitivity to antibiotics, especially Imipinem, Ciprofloxacin. The extraction method depends mainly on the presence of sodium cholate and EDTA and then using chromatography purification by gel filtration (Sephacryl S-200) Then the molecular weight of the outer membrane proteins (porins) determined using electrophoresis under denaturing conditions. The molecular weight of the protein was 48 and 25 KDa, respectively, for Porins of P. aeruginosa isolated from wounds and Porins of P. fluorescens isolated from otitis media in the final product, the highest concentration of the outer membrane proteins (porins) partially purified from P. aeruginosa and P. fluorescens was 1.92, 1.32 mg/ ml, respectively