Detection of Mycoplasma hominis and Ureaplasma Urealyticum in urethritis from sexual active men by Real-time PCR


Background: Mycoplasma spp and Ureaplasma spp are unique microbes in they lack a cell wall and cause genitourinary tract infections ,these organisms are considered an intracellular parasites and difficult to cultivate in routine media due to the complexity of their nutritional requirements so , the molecular detection and identify these microorganisms by Real-Time PCR due to cost, time, reliability, specificity, and sensitivity.Aim of study : To detect and differentiate Mycoplasma hominis and Ureaplasma urealyticum isolates from patients with urethritis by Real-Time PCR.Methods: Ureathral swabs collected from 60 sexual active men from December 2012 to May 2013 who attending to AL- Diwanyia Teaching Hospital were tested by using DUPLICα RealTime PCR kit for the presence the of genital mycoplasmas (Mycoplasma hominis based on amplification of 16SrRNA gene and Ureaplsma uralyticum based on amplification of urease gene ) , the age of patients study with urethritis were (18-41) year old. . Results: Out of 60 Urethral samples there 28 were positive samples as 3 (10.7%) , 17 (60.7%) and 5 (17.8%) for M.hominis (as a single pathogen) ,U.urealyticum (as a single pathogen) an both of genital mycoplasma respectively the age group (30-35) years old was more significant (P<0.05) than other group ages08Conclusion :Real-PCR shows a rapid and high accuracy in detection and differentiation of M. hominis and U. urealyticum from clinical samples. Keywords: Mycoplasma , Ureaplasma , Real-Time PCR , Urethritis