Isolation and identification of Fusarium oxysporum and Aspergillus fumigates from blood specimens in iraq and study efficiency of some plant essential oils

Abstract

Ten blood samples were collected from immunocompromised patients. Fusarium oxysporum, Aspergillus fumigatus were isolated and identified based on morphological characteristics which include hyphae, macroconidia, microconidia, conidiophores, conidial color, vesicle, matulae and phialides. Five isolates of F. oxysporum ( F1-12, F2-13, F3-14, F4-15, F5-16 ) and four isolates of A. fumigates (AS1, AS2, AS3, AS4) were identified. Five types of plant essential oils, peppermint oil, clove oil, cardamom oil, chamomile oil and castor oil were used. Results shown that all plant essential oils at different concentrations inhibit growth of F. oxysporum F5-16. Peppermint oil, olive oil and castor oil at highest concentration (35mg/ml) caused highest reduction of mycelail growth (100%) and same at highest concentration (35mg/ml), peppermint, cardamom and chamomile was found most effective (83.4-100%) in reducing mycelia growth of A. fumigates AS1, compared with the control.