Characterization of phenolic compounds in the bottom beauty flower leaf flowers Hibiscus rosa-sinensis HPLC technology and the study of its impact on the level of estrogen and fertility of male rats eggs.

Abstract

This study was conducted to investigate the effect of phenolic extract of flowers beauty leaf flower Hibiscus rosa-sinensis in estrogen and fertility level of male rats eggs, as well as in the characterization of phenolic compounds extracted using Kromatokravaa liquid technology of high performance HPLC. 36 rats were used white adult, at 12 y cr and 24 male Female divided into two groups each containing 6 rats were fed to the first group at a dose of 300 mg / kg bw / day for 60 days, and I went back the second control group. It was paired all the males from both groups a pair of female Tmanah for two weeks. The results showed for the increase of moral P <0.05)) in the level of estrogen to male rats Elmejrah compared with the control group. As the results show for a significant decrease P <0.05)) in the percentage of pregnancies in female pairing with males treatment as there has been no pregnancy until the sixth week confused, the pregnancy rate of 16% and reached the seventh week and 50% in the eighth week of confusion, compared with the control group, which amounted to pregnancy where the ratio of 100% in the second week. The results also showed six of the vehicles in the diagnosis of phenolic extract of the plant blossoms responded beauty using HPLC method are: acid steel Salsellk Acetysalisaic acid, acid Alkruceng Chlorogenic acid and coumarin Coumarin and lutein Lutein and Saandin Cynidin and Korosten Quercetin.