Isolation and Diagnosis species related to Stachybotrys from Indoor Buildings in AL- Najaf Province and its ability to produce verrucarol toxin

Abstract

Two species of Stachybotrys are isolated which are S. chartarum and S. echinata. It was observed that S. chartarum was more frequency than other fungi on wheat straw medium and reached to 82.5%.Also Stachybotrys is growing on materials which rich in cellulose such as straw medium.Stachybotrys preferred to grow on the gypsum powder and reaches to 78.8% in these materials and components which supporting fungal growth.HPLC results show that retention time or major peak of standard verrucarol toxin are located at 5.6 minutes from 4.5-6.5which identifies with toxin verrucarol produced from a filter extract of S. chartarum in the same area.