Determination of genetic diversity of Iraqi date palm (Phoenix dactylifera L.) by using ISSR technique

Abstract

This study provided that all molecular markers inter simple equence repeat (ISSR) succeded with the sixty five Iraqi date palm (Phoenix dactylifera L.) cultivars, which collected from Hilla city in Iraq, to determine fingerprinting, polymorphic value, and genetic relationships among varieties of date palm cultivars, and also with the same type of cultivars. This study was used ten of ISSR primers from different sources and showed the average percentage of polymorphism per ISSR-primer was %98.9, primer efficiency was %0.13 and discrimination value was %0.09. This study revealed that high similarity percentage was about %67 to %100 between cultivars belonged to the same variety. Genetic similarty with twenty Iraqi date palm cultivars, using UBC-835 primer as a sample in this study revealed the highest distance was 0.5345 between Breem and each of varieties (SabbDrrh, Khadrawi, FomAlraman, shwrthi and GhanamiAhmer), that means no similarity with these date palm cultivars. On the other hands, the lowest genetic distance was (zero) between Tebrazal and each of (Maktom and Najdi). Also between SabbDrrah and each of (Khadrawi, FomAlrman, Shwethi, and GhanamiAhmer). In addition to that between Hamrawi and each of (Kestawi and Chipchab). There are nogentic distance with Brban and Sukkary. Also between Khadrawi and each of (FomAlrman, Shwethi and GhanamiAhmer).Additionaly,no genetic distance between Khadrawi and Chipchab, Maktom and Najdi. Also, no genetic distance between FomAlraman and each of Shwethi, and GhanamiAhmer). At the end no genetic distance between Shwethi, and GhanamiAhmer. No distance between these varieties was observed.