The Activity of α-Amylase Enzyme in the Gut Extract of Three Fish Species in the Southern Part of Chybaesh Marsh, Iraq


The study aim to estimation the activity of α-Amylase enzyme in the gutextract of three fish species with varying in their feeding habit i.e. Shilluk Leuciscusvorax (carnivores), blue tilapia Oreachromis aureus (herbivores) and Khushani Lizaabu (omnivores) in the Southern part of Chybaesh Marsh, Iraq for the period from April(2014) to December in (2015). The fishes were collected by gill nets and electricalfishing apparatus. A sample, part from the gut of each species of fish were taken in thefield and placed plastic tube (10)ml and then preserved in a container containing liquidnitrogen (-190C°). To estimating of the enzyme activity a kit was used (Amylasemethod E-PNPG7). The results showed superiority of herbivores fish, which reachedthe maximum activity of the enzyme in autumn (550.65 IU / g tissue) while the lowestvalue recorded in spring (176.28 IU / g tissue), the statistical analysis showedsignificant differences (sig= 0.0017, p˃0.05 ) between tilapia and khushni, tilapia andshilluk (sig=0.0025, p˂0.05) ,while not recorded significant differences (sig= 0.0887,p˃0.05) between the shilluk and the Khushni,whereas recorded between the omnivoresand carnivores, also positive significant correlation was found between enzyme activityin Khushani and water temperature (r = 0.717) but thereis not significant differences (r= 0.467, r = -0.337) between temperature of Shilluk and tilapia respectively.