The Antibacterial effect of extracts of Salvadora Perscia against oral pathogenic bacteria isolated from dental caries

Abstract

background and aims: Most pathogenic bacteria became resistance to antibiotic which leads to failure in treatment of bacterial infections by antibiotics , wherefore in the recent time go toward the medical alternative treatment of bacterial infections, therefore this present investigation aimed to evaluated efficient of extract of Salvadora Perscia (miswak) as antibacterial (an alternate to antibiotics)against six bacterial species: Staphylococcus.aureus , Staphylococcus.faecalis, Staphylococcus.mutans, Staphylococcus.pyogenes (Group A streptococcus), Pseudomonas aeruginosa and Escherichia coli) isolated from dental caries with study the antibiotic resistant to these bacterial isolate and comparative between them.Method: in this study using aqueous and methanol extract of Salvadora persica against bacterial isolates from dental caries after diagnostic bacteria by culturing on media (macConkey and blood) agar, classical methods and Vitek system with study the antibiotic resistant pattern to these bacterial isolate by disc agar diffusion.Results: This investigated founded that aqueous extracts of Salvadora persica was high activity at (10000µg/ ml) against all tested bacteria while weakest activity at ( 500µg/ ml ) on Staphylococcus.aureus , Staphylococcus. faecalis and Escherichia coli (Inhibition Zone (I.Z) =6.5 and 8) mm respectively, whilst methanol extracts of Salvadora persica strong activity at (10000µg/ ml) against Pseudomonas.aeruginosa, streptococcus.mutans as well as both Escherichia coliand streptococcus. pyogenes (Group A streptococcus)( I.Z =17, 16 and 15 ) mm respectively, followed by Staphylococcus.Pyogenen in both concentration (5000, 1000µg/ ml) (I.Z =12 mm) and weaker activity in (500µg/ ml) against Staphylococcus.faecalis and Staphylococcus.aureus (I.Z =4, 6) mm respectively , whilst antibiotics sensitivity study showed streptococcus.faecalis sensitive to Ciprofloxacin,Gentamycin , Norfloxacin and Streptomycin (I.Z =18,17,18,15) mm respectively and this bacteria appearance resistance for both Amoxicillin and Chloramphenicol ( I.Z= 7mm) as well as some of isolates as staphylococcus.aureus and Pseudomonas.aeruginosa were resistant to both Chloramphenicol and Gentamycin (I.Z = 5.5)mm respectively whilst streptococcus.pyogenes (Group A streptococcus), streptococcus.mutans and Escherichia coli were sensitivity to Chloramphenicol , Ciprofloxacin and Gentamycinas (22, 20 and 21) mm respectively.Conclusion: Salvadora persica can be used as antibacterial.