RT-PCR detection of SAP1 gene in Candida albicans isolated from Meningitis cases

Abstract

Objective : The main objective of the sampling is to isolate fungi associated with cases of meningitis ,study of some Virulence factors of fungi and sensitivity to antifungal agents. Methods: 100 CSF sample were suspected of meningitis culture ,biochemical test and API-System for diagnosis of fungi in sample ,Real-time PCR based on SYBR Green I fluorescent dye for detection of sap gene in Candida albicans. Results: Depending on agricultures characteristics ,Biochemical tests and Api System the results showed that 32 samples, 32% were given the positive result for yeast growth, Candida albicans found in these samples by 87.5% and Cryptococcus neoformans 12.5% and 25(89.28%) of C.albicans isolates had Sap gene. C. albicans showed the lowest MICs (1 μg/ml) for amphotericin B. and higher MICs for fluconazole (4 - 32 μg/ml) and Nystatin (8-32 μg/ml) While C.neoformans showed low MICs for all antifungal agents used in this study ranged from (0.06-0.5 μg/ml) for amphotericin B and ( 0.25 - 1 μg/ml) for fluconazole and ( 1-4 μg/ml) for nystatin Conclusion: Fungal Meningitis which caused by Candida albicans which have SAP gene that consider as virulence factors and Resistant to antifungal agents and Cryptococcus neoformans is a debilitating and potentially deadly disease that affects patients with both intact and impaired immune systems. early diagnosis and treatment is essential for optimal outcomes. Patients with Fungal Meningitis have multisystem disturbances and require a well-organized and executed plan of care.