A Comparative Evaluation of Apical Seal Associated with Ultrasonic Retrograde Cavities Filled with Bioactive Material (In Vitro Study)


Background: The aim of this study was to evaluate and compare the apical microleakage around retrogradecavities prepared with ultrasonic technique and filled with (Biodentine™)Materials and methods: 40 extracted single rooted human permanent maxillary teeth with mature apices wereselected. The roots were prepared chemo-mechanically using k-files with crown-down technique and thenobturated with lateral condensation gutta-percha technique. Teeth were divided into four main groups according tothe cavity preparation method either manual or ultrasonic technique:Group A (n=10): A class I retrograde cavity at root end was prepared with traditional handpeice equipped andplacement of Biodentine with manual condensation.Group B (n=10): A class I retrograde cavity at root end was prepared with Piezoelectric ultrasonic device equippedwith ultrasonic tip with only manual compaction of the material.Group C (n=10): traditional handpeice and placement of Biodentine using both manual compaction and 5 secondultrasonic activation.Group D (n=10): Piezoelectric ultrasonic device and placement of Biodentine with both manual compaction and 5second ultrasonic compaction. The teeth were immersed in 1% aqueous Methylene blue dye for 72 hr. Then theywere sectioned longitudinally with a diamond disc and the depth of dye penetration was examined under highmagnification 20X.Results: Statistical analysis showed a highly significant difference in microleakage among the tested groups in whichPiezoelectric technique has proved superiority in retrograde cavity preparation and compaction of Biodentin whendye penetration scores were compared. So microleakage was highest with cavities prepared with handpeice andmanual application of retrograde material group A (2.73±0.39) followed by microprepared cavities group C(1.86±0.16), and it was lowest with ultrasonically-prepared cavities group B (1.09±0.28) and group D (0.26±0.19).Conclusion: ultrasonic preparation produced significantly less microleakage than conventional method. Also lessmicroleakage was observed with ultrasonic compaction of Biodentin when compared with conventional method ofcompaction.