Assessment of Alkaline Phosphatase, Salivary Flow Rate and Salivary Potential of Hydrogen in Relation to Severity of Chronic Periodontitis

Abstract

Background: The cells of periodontium contain many intracellular enzymes like (alkaline phosphatase ALP) that arereleased outside into the saliva and gingival crevicular fluid (GCF) after destruction of periodontal tissue. The aim ofstudy was to determine the activity of this enzyme in saliva and its relation to the salivary flow rate, PH and clinicalperiodontal parameters in patients with chronic periodontitis.Subject, Materials and methods: Sample population consist of 75 individuals ;divided into four groups , the first group(15):control subject, the second group (20):mild chronic periodontitis, the third group(20) moderate chronicperiodontitis and the fourth group (20) sever chronic periodontitis, Measurements of plaque index (PLI), gingival index(GI), bleeding on probing (BOP), probing pocket depth (PPD) and clinical attachment level (CAL), only male wereincluded and saliva was collected from them and subjected to biochemical analysis of the alkaline phosphataseenzyme (ALP), and also measurement of salivary flow rate(FR) and PH.Results: Statistical analysis of the results revealed the presence of a highly significant difference in the enzymaticactivity between healthy and chronic periodontitis subjects (mild, moderate, severe) with positive correlationbetween the activity of this enzyme and the clinical periodontal parameters, and negative correlation between thisenzyme and Salivary flow rate and pH.Conclusion: From this study it can be concluded that a number of markers show promise as sensitive measures ofdisease and the effectiveness of therapy. At this time enzymes such as alkaline phosphatase ALP, is goodbiochemical markers of screening chronic periodontitis. Also ALP can be used as a monitor for healthy individualsand patients with different periodontal diseases. Furthermore, analysis of saliva may offer a cost effective approachto assessment in controlling progression of chronic periodontitis in large populations