Antibacterial Effect of Aqueous and Alcoholic Propolis Extracts on Aggregatibacter Actinomycetemcomitans in Patients with Chronic Periodontitis (An In-vitro Study)


Background: Propolis has received great interest because of its wide range antimicrobial activity. Propolis also called(bee glue) due to its collection by (Apismellifera) honeybees from various plants resinous substance. The aim of thisstudy was to determine the antibacterial effect of propolis extracts (aqueous and alcoholic) on anaerobicperiodontal pathogen namely Aggregatibacteractinomycetemcomitans.Materials and Methods: Strains of Aggregatibacter actinomycetemcomitans wasisolated from pockets ofsystemically healthy patients aged between 35-55 years old suffering from chronic periodontitis with pocket depthsof 5-6 mm, the bacteria cultured on special blood Agar plates solid media. Propolis was extracted by using waterand alcohol. Agar well technique was used to study the sensitivity of Aggregatibacter actinomycetemcomitans todifferent concentrations of propolis extracts (70, 80, 90, 100, 125 and 150) mg/ml and other control agents (distilledwater and chlorhexidine 0.2%).Results: Aggregatibacter actinomycetemcomitans was sensitive to propolis extracts; alcoholic extract was moreeffective than aqueous extract.All concentrations of propolis extracts showed smaller inhibition zones than 0,2% CHXexcept 150 mg/ml concentration of aqueous extract ,(100, 125 and 150)mg/ml concentrations of alcoholic extractshowed larger inhibition zones than 0,2% CHX.Conclusion: Propolis extracts were effective against anaerobic periodontal pathogens (Aggregatibacteractinomycetemcomitans).