Estimation of Interferon-α (IFN-α) and Tumor Necrosis Factor-α (TNF-α) in Female Rats Immunized by Human Breast Cancer Cell Line T47D


Breast cancer is the greatest frequently cancer that occurs in women. This study was carried out on different groups of female albino rats Rattus rattus, that are immunized by different forms of antigens prepared from a cell line of human breast cancer T47d. The current study has been designed to determine the immune response of rats for breast cancer cells antigens. The immune parameters IFN-α and TNF-α have been estimated. The results have shown the existence of a significant increase in the level of IFN-α in all treated groups compared with two control groups, and higher level shown in groups of rats that are injected by freezing antigen mixed with adjuvant, heating killed antigen and chemical killed antigen compared with other groups, which reached to 1.783±0.143,1.766±0.0.183 and 1.708±0.175 (ng/ml) respectively. The level of TNF-α reveals a significant increase in the treatment groups compared with control groups, and the highest level has been in groups of rats injected by freezing killed antigen mixed with an adjuvant and also by chemical killed antigen with an adjuvant which reached to 0.265±0.045 and 0.242±0.067 (ng/ml) respectively.