Effects of sorbitol fermenting STEC (O157: H-) in experimentally infected mice on blood urea and histology of colon and kidney

Abstract

Shiga toxin producing E. coli (STEC) are an important zoonotic disease and a food contaminated pathogens of human prompting serious illness in gastrointestinal tract such as hemorrhagic colitis and intense renal failure. This study was intended to research the influence of tentatively infected mice with STEC O157: H- on blood plasma urea and histopathological changes in colon and kidney. STEC Strain, which has been used in this study was (O157: H- positive to both stx1 and stx2) by PCR analysis, Sixty male mice ranged from 8 to 12 weeks old, were partitioned haphazardly into two groups (n=30), first one (infected group) were orally inoculated with (10 10 CFU/ml in PBS), while the second gathering (control group) was gotten PBS orally. Three, seven and fourteen days post infection, blood were collected from 10 animal to each group by cardiac puncture to measure the blood plasma urea, then the colon and kidney were removed for histopathological analysis to recognize the influence of contamination on these organs. The results demonstrated huge expand (P<0.05) in infected at contrasted with a control set at all experimental period meanwhile infected group showed a significantly increased (P<0.05) in urea level at day 7 and 14 in contrast with day 3 of a test. The histopathological investigation includes; colon and kidney illustrated mild diffuse inflammation which includes expanded inflammatory cells in tissue intraepithelial and goblet cells hyperplasia. Renal tubules showed contracting lumen with epithelial cell extensive injured, swelling and generalized necrotic characterized by acute tubular necrosis. The study concluded that tentatively contaminated mice with isolated strain (O157: H-) from children showed elevated of blood plasma significantly urea after infection, with sever pathological changes in colon and kidney at all time of investigation portrayed by sever colitis and intense kidney necrosis